Mouse Kiss1 ELISA Kit (2) (V2LY-0626-LY2651)

Name: Mouse Kiss1 ELISA Kit (2)
SKU: V2LY-0626-LY2651
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0035 ng/mL

Detection Range

0.007-1.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

14.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

KiSS-1 Metastasis Suppressor

Function

Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.

Biological Process

Cytoskeleton organizationManual Assertion Based On ExperimentTAS:ProtInc
G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Generation of ovulation cycle rhythmIEA:Ensembl
Negative regulation of cell population proliferationIEA:Ensembl
Positive regulation of cytosolic calcium ion concentrationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathwayIEA:Ensembl
Positive regulation of growth hormone secretionIEA:Ensembl
Positive regulation of luteinizing hormone secretionIEA:Ensembl
Positive regulation of MAPK cascadeIEA:Ensembl
Positive regulation of synaptic transmissionIEA:Ensembl

Cellular Location

Secreted

Involvement in disease

Hypogonadotropic hypogonadism 13 with or without anosmia (HH13):
A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. In some cases, it is associated with non-reproductive phenotypes, such as anosmia, cleft palate, and sensorineural hearing loss. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In the presence of anosmia, idiopathic hypogonadotropic hypogonadism is referred to as Kallmann syndrome, whereas in the presence of a normal sense of smell, it has been termed normosmic idiopathic hypogonadotropic hypogonadism (nIHH).

PTM

Processed by MMP2 and MMP9.

Li, Z., Liu, J., Inuzuka, H., & Wei, W. (2022). Functional analysis of the emerging roles for the KISS1/KISS1R signaling pathway in cancer metastasis. Journal of Genetics and Genomics, 49(3), 181-184.

Ly, T., Harihar, S., & Welch, D. R. (2020). KISS1 in metastatic cancer research and treatment: potential and paradoxes. Cancer and Metastasis Reviews, 39(3), 739-754.

Talbi, R., & Navarro, V. M. (2020). Novel insights into the metabolic action of Kiss1 neurons. Endocrine connections, 9(5), R124-R133.

Ikegami, K., Goto, T., Nakamura, S., Watanabe, Y., Sugimoto, A., Majarune, S., ... & Uenoyama, Y. (2020). Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice. Journal of Reproduction and Development, 66(4), 359-367.

Ulasov, I. V., Borovjagin, A. V., Timashev, P., Cristofanili, M., & Welch, D. R. (2019). KISS1 in breast cancer progression and autophagy. Cancer and Metastasis Reviews, 38, 493-506.

Yeo, S. H., Kyle, V., Blouet, C., Jones, S., & Colledge, W. H. (2019). Mapping neuronal inputs to Kiss1 neurons in the arcuate nucleus of the mouse. PloS one, 14(3), e0213927.

Guzman, S., Brackstone, M., Radovick, S., Babwah, A. V., & Bhattacharya, M. M. (2018). KISS1/KISS1R in cancer: friend or foe?. Frontiers in endocrinology, 9, 437.

Vazquez, M. J., Toro, C. A., Castellano, J. M., Ruiz-Pino, F., Roa, J., Beiroa, D., ... & Tena-Sempere, M. (2018). SIRT1 mediates obesity-and nutrient-dependent perturbation of pubertal timing by epigenetically controlling Kiss1 expression. Nature communications, 9(1), 4194.

Kostakis, I. D., Agrogiannis, G., Vaiopoulos, A. G., Mylona, E., Patsouris, E., Kouraklis, G., & Koutsilieris, M. (2018). KISS1 and KISS1R expression in gastric cancer. J buon, 23(01), 79-84.

Chakravarthi, V. P., Khristi, V., Ghosh, S., Yerrathota, S., Dai, E., Roby, K. F., ... & Rumi, M. K. (2018). ESR2 is essential for gonadotropin-induced Kiss1 expression in granulosa cells. Endocrinology, 159(11), 3860-3873.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Kiss1 ELISA Kit (CAT#: V2LY-0626-LY1252)

Mouse Kiss1 ELISA Kit (CAT#: V2LY-0626-LY1427)

Human KISS1 ELISA Kit (CAT#: V2LY-0626-LY3635)

Human KISS1 ELISA Kit (2) (CAT#: V2LY-0626-LY4884)

KISS1 Matched Antibody Pair (667) (CAT#: APMAB-667LY)

Antibody Pairs

KISS1 Matched Antibody Pair (667) (CAT#: APMAB-667LY)

Related Products

Mouse Anti-KISS1 Recombinant Antibody (1F7) (CAT#: CBMAB-K0955-LY)

KISS1 Matched Antibody Pair (667) (CAT#: APMAB-667LY)

Mouse Kiss1 ELISA Kit (CAT#: V2LY-0626-LY1427)

Human KISS1 ELISA Kit (CAT#: V2LY-0626-LY3635)

Mouse Anti-KISS1 Recombinant Antibody (A766) (CAT#: CBMAB-AP4162LY)

Human KISS1 ELISA Kit (2) (CAT#: V2LY-0626-LY4884)

Rat Kiss1 ELISA Kit (CAT#: V2LY-0626-LY1252)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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