Mouse Klk1 ELISA Kit (2) (V2LY-0626-LY2496)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.014 ng/mL
Detection Range
0.03-9 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
28.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
kallikrein 1
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Biological Process
Regulation of systemic arterial blood pressureManual Assertion Based On ExperimentIBA:GO_Central
Zymogen activationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Extracellular exosome
Nucleus
Secretory granule
PTM
The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.

Iwasaki, T., Tokumori, M., Matsubara, M., Ojima, F., Kamigochi, K., Aizawa, S., ... & Takahashi, S. (2023). A regulatory mechanism of mouse kallikrein 1 gene expression by estrogen. Molecular and cellular endocrinology, 577, 112044.

Zhang, M., Luo, C., Lin, D., Cui, K., Chen, Z., & Liu, J. (2021). Human tissue kallikrein 1 is downregulated in elderly human prostates and possesses potential in vitro antioxidative and antifibrotic effects in rodent prostates. Oxidative Medicine and Cellular Longevity, 2021.

Ma, L., Wu, J., Zheng, Y., Shu, Z., Wei, Z., Sun, Y., ... & Zhou, A. (2020). Heparin blocks the inhibition of tissue kallikrein 1 by kallistatin through electrostatic repulsion. Biomolecules, 10(6), 828.

Adamopoulos, P. G., Kontos, C. K., & Scorilas, A. (2019). Discovery of novel transcripts of the human tissue kallikrein (KLK1) and kallikrein-related peptidase 2 (KLK2) in human cancer cells, exploiting Next-Generation Sequencing technology. Genomics, 111(4), 642-652.

Devetzi, M., Goulielmaki, M., Khoury, N., Spandidos, D. A., Sotiropoulou, G., Christodoulou, I., & Zoumpourlis, V. (2018). Genetically‑modified stem cells in treatment of human diseases: Tissue kallikrein (KLK1)‑based targeted therapy. international journal of molecular medicine, 41(3), 1177-1186.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant KLK1 protein, His Tag (CAT#: V2LY-0526-LY5117)

Human Recombinant KLK1 protein, His Tag-1 (CAT#: V2LY-0526-LY5118)

Mouse Recombinant KLK1 protein, His Tag (CAT#: V2LY-0526-LY8583)

Human KLK1 ELISA Kit (CAT#: V2LY-0626-LY1126)

Mouse Klk1 ELISA Kit (CAT#: V2LY-0626-LY1640)

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Human Recombinant KLK1 protein, His Tag (CAT#: V2LY-0526-LY5117)

Mouse Anti-KLK1 Recombinant Antibody (3G2) (CAT#: CBMAB-K1205-LY)

Mouse Recombinant KLK1 protein, His Tag (CAT#: V2LY-0526-LY8583)

Mouse Anti-KLK1 Recombinant Antibody (CBLG1-2325) (CAT#: CBMAB-G6184-LY)

Mouse Klk1 ELISA Kit (CAT#: V2LY-0626-LY1640)

Human Recombinant KLK1 protein, His Tag-1 (CAT#: V2LY-0526-LY5118)

Mouse Anti-KLK1 Recombinant Antibody (10G3-42) (CAT#: CBMAB-P0425-YC)

Rabbit Anti-KLK1 Recombinant Antibody (105) (CAT#: CBMAB-K1203-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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