Mouse Mc5r ELISA Kit (V2LY-0626-LY2619)

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Tested Data
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0096 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
37.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
MELANOCORTIN 5 RECEPTOR
Function
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: ProtInc
Regulation of metabolic process Source: GO_Central
Cellular Location
Cell membrane
Topology
Extracellular: 1-37
Helical: 38-61
Cytoplasmic: 62-73
Helical: 74-97
Extracellular: 98-114
Helical: 115-138
Cytoplasmic: 139-155
Helical: 156-179
Extracellular: 180-186
Helical: 187-211
Cytoplasmic: 212-239
Helical: 240-265
Extracellular: 266-273
Helical: 274-297
Cytoplasmic: 298-325

Feng, W., Zhou, Q., Chen, X., Dai, A., Cai, X., Liu, X., ... & Wang, M. W. (2023). Structural insights into ligand recognition and subtype selectivity of the human melanocortin-3 and melanocortin-5 receptors. Cell Discovery, 9(1), 81.

Ji, L. Q., Hong, Y., & Tao, Y. X. (2022). Melanocortin-5 receptor: Pharmacology and its regulation of energy metabolism. International Journal of Molecular Sciences, 23(15), 8727.

Xu, Y., Guan, X., Zhou, R., & Gong, R. (2020). Melanocortin 5 receptor signaling pathway in health and disease. Cellular and Molecular Life Sciences, 77, 3831-3840.

Dores, R. M., Oberer, N., Hoglin, B., Thomas, A., Faught, E., & Vijayan, M. M. (2020). Evaluating interactions between the melanocortin-5 receptor, MRAP1, and ACTH (1–24): A phylogenetic study. General and Comparative Endocrinology, 294, 113476.

Zhu, M., Wang, M., Chen, Y., & Zhang, C. (2019). Pharmacological modulation of two melanocortin-5 receptors by MRAP2 proteins in zebrafish. Journal of Molecular Endocrinology, 62(1), 27-36.

Liao, S., Dong, J., Xu, W., Xi, B., Tao, Y., Liu, B., & Xie, J. (2019). Molecular cloning, tissue distribution, and pharmacological characterization of blunt snout bream (Megalobrama amblycephala) melanocortin-5 receptor. Fish physiology and biochemistry, 45, 311-321.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human MC5R ELISA Kit (CAT#: V2LY-0626-LY3808)

Related Products

Mouse Anti-MC5R Recombinant Antibody (CBFYM-0213) (CAT#: CBMAB-M0256-FY)

Human MC5R ELISA Kit (CAT#: V2LY-0626-LY3808)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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