Mouse Recombinant CD302 protein, His Tag (V2LY-0526-LY7995)

Name: Mouse Recombinant CD302 protein, His Tag
SKU: V2LY-0526-LY7995
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CD302 protein, His Tag consist of Amino Acid: 1-156 and predicts a molecular mass of 17 kDa.

Molecule Mass

17 kDa

Sequence

Amino Acid: 1-156

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>93% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl, Gly

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD302 molecule

Function

Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration.

Biological Process

Phagocytosis

Cellular Location

Membrane
Cell projection, filopodium
Cytoplasm, cell cortex
Cell projection, microvillus

Topology

Extracellular: 23-168
Helical: 169-189
Cytoplasmic: 190-232

PTM

May be heterogeneously N-glycosylated in some cell types.

Brown, R. J., Tegtmeyer, B., Sheldon, J., Khera, T., Anggakusuma, Todt, D., ... & Pietschmann, T. (2020). Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice. Science advances, 6(45), eabd3233.

Kok, C. H., Liu, L., Yeung, D. T., Saunders, V. A., Dang, P., & Hughes, T. P. (2019). A Combination of CD302 gene Expression and 3-Months BCR-ABL1 Level Predicts Inferior Achievement of Deep Molecular Response in CP-CML Patients Treated with Imatinib.

Lo, T. H., Abadir, E., Gasiorowski, R. E., Kabani, K., Ramesh, M., Orellana, D., ... & Clark, G. J. (2019). Examination of CD302 as a potential therapeutic target for acute myeloid leukemia. Plos one, 14(5), e0216368.

Alaterre, E., Raimbault, S., Goldschmidt, H., Bouhya, S., Requirand, G., Robert, N., ... & Moreaux, J. (2017). CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma. Oncotarget, 8(58), 98931.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CD302 protein, hFc Tag (CAT#: V2LY-0526-LY7994)

Rat Recombinant CD302 protein, hFc Tag (CAT#: V2LY-0526-LY9230)

Rat Recombinant CD302 protein, His Tag (CAT#: V2LY-0526-LY9231)

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Mouse Anti-CD302 Recombinant Antibody (C9214) (CAT#: CBMAB-C9214-LY)

Rat Recombinant CD302 protein, His Tag (CAT#: V2LY-0526-LY9231)

Rat Recombinant CD302 protein, hFc Tag (CAT#: V2LY-0526-LY9230)

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Mouse Anti-CD302 Recombinant Antibody (771910) (CAT#: CBMAB-C8529-LY)

Mouse Anti-CD302 Recombinant Antibody (CBT3819) (CAT#: V2LY-0625-LY1147)

Mouse Recombinant CD302 protein, hFc Tag (CAT#: V2LY-0526-LY7994)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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