Mouse Recombinant CHST3 protein, His Tag (V2LY-0526-LY8112)

Name: Mouse Recombinant CHST3 protein, His Tag
SKU: V2LY-0526-LY8112
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CHST3 protein, His Tag consist of Amino Acid: 39-472 and predicts a molecular mass of 52 kDa.

Molecule Mass

52 kDa

Sequence

Amino Acid: 39-472

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

carbohydrate (chondroitin 6) sulfotransferase 3

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.

Biological Process

Carbohydrate metabolic process Source: UniProtKB-KW
Chondroitin sulfate biosynthetic process Source: UniProtKB
N-acetylglucosamine metabolic process Source: GO_Central
Sulfur compound metabolic process Source: UniProtKB

Cellular Location

Golgi apparatus membrane

Involvement in disease

Spondyloepiphyseal dysplasia with congenital joint dislocations (SEDCJD):
A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood.

Topology

Cytoplasmic: 1-20
Helical: 21-38
Lumenal: 39-479

Guan, Y., Sun, C., Zou, F., Wang, H., Lu, F., Song, J., ... & Ma, X. (2021). Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus. Journal of Cellular and Molecular Medicine.

Albuz, B., Çetin, G. O., Özhan, B., Sarikepe, B., Anlas, Ö., Öztürk, M., ... & Gündüz, C. N. S. (2020). A novel nonsense mutation in CHST3 in a Turkish patient with spondyloepiphyseal dysplasia, Omani type. Clinical dysmorphology, 29(1), 61-64.

Baidoe-Ansah, D., Sakib, M. S., Jia, S., Fischer, A., Kaushik, R., & Dityatev, A. (2019). Epigenetic mechanism of carbohydrate sulfotransferase 3 (CHST3) downregulation in the aging brain. bioRxiv, 741355.

Superti-Furga, A., & Unger, S. (2019). CHST3-related skeletal dysplasia.

Feng, L., Li, Y., Li, Y., Jiang, Y., Wang, N., Yuan, D., & Fan, J. (2018). Whole exome sequencing detects CHST3 mutation in patient with acute promyelocytic leukemia: a case report. Medicine, 97(36).

Wang, L., Guo, X., Yi, J., Qu, C., Lei, J., Guo, Q., & Han, J. (2018). The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats. Journal of Trace Elements in Medicine and Biology, 50, 123-129.

Yorifuji, K., Uemura, Y., Horibata, S., Tsuji, G., Suzuki, Y., Miyagawa, K., ... & Emoto, N. (2018). CHST3 and CHST13 polymorphisms as predictors of bosentan-induced liver toxicity in Japanese patients with pulmonary arterial hypertension. Pharmacological research, 135, 259-264.

Muys, J., Blaumeiser, B., Jacquemyn, Y., & Janssens, K. (2017). Prenatal homozygosity mapping detects a novel mutation in CHST3 in a fetus with skeletal dysplasia and joint dislocations. Clinical case reports, 5(4), 440.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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