Mouse Anti-CHST3 Recombinant Antibody (1D3) (CBMAB-A1621-LY)

Name: Mouse Anti-CHST3 Recombinant Antibody (1D3)
SKU: CBMAB-A1621-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D3

Application

WB, ELISA

Immunogen

CHST3 (312 a.a. ~ 411 a.a) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

carbohydrate (chondroitin 6) sulfotransferase 3

Introduction

This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq]

Entrez Gene ID

9469

UniProt ID

Q7LGC8

Alternative Names

C6ST; C6ST1; HSD

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.

Biological Process

Carbohydrate metabolic process Source: UniProtKB-KW
Chondroitin sulfate biosynthetic process Source: UniProtKB
N-acetylglucosamine metabolic process Source: GO_Central
Sulfur compound metabolic process Source: UniProtKB

Cellular Location

Golgi apparatus membrane

Involvement in disease

Spondyloepiphyseal dysplasia with congenital joint dislocations (SEDCJD):
A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood.

Topology

Cytoplasmic: 1-20
Helical: 21-38
Lumenal: 39-479

Guan, Y., Sun, C., Zou, F., Wang, H., Lu, F., Song, J., ... & Ma, X. (2021). Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus. Journal of Cellular and Molecular Medicine.

Albuz, B., Çetin, G. O., Özhan, B., Sarikepe, B., Anlas, Ö., Öztürk, M., ... & Gündüz, C. N. S. (2020). A novel nonsense mutation in CHST3 in a Turkish patient with spondyloepiphyseal dysplasia, Omani type. Clinical dysmorphology, 29(1), 61-64.

Baidoe-Ansah, D., Sakib, M. S., Jia, S., Fischer, A., Kaushik, R., & Dityatev, A. (2019). Epigenetic mechanism of carbohydrate sulfotransferase 3 (CHST3) downregulation in the aging brain. bioRxiv, 741355.

Superti-Furga, A., & Unger, S. (2019). CHST3-related skeletal dysplasia.

Feng, L., Li, Y., Li, Y., Jiang, Y., Wang, N., Yuan, D., & Fan, J. (2018). Whole exome sequencing detects CHST3 mutation in patient with acute promyelocytic leukemia: a case report. Medicine, 97(36).

Wang, L., Guo, X., Yi, J., Qu, C., Lei, J., Guo, Q., & Han, J. (2018). The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats. Journal of Trace Elements in Medicine and Biology, 50, 123-129.

Yorifuji, K., Uemura, Y., Horibata, S., Tsuji, G., Suzuki, Y., Miyagawa, K., ... & Emoto, N. (2018). CHST3 and CHST13 polymorphisms as predictors of bosentan-induced liver toxicity in Japanese patients with pulmonary arterial hypertension. Pharmacological research, 135, 259-264.

Muys, J., Blaumeiser, B., Jacquemyn, Y., & Janssens, K. (2017). Prenatal homozygosity mapping detects a novel mutation in CHST3 in a fetus with skeletal dysplasia and joint dislocations. Clinical case reports, 5(4), 440.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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