Human CHST3 ELISA Kit (V2LY-0626-LY3499)

Name: Human CHST3 ELISA Kit
SKU: V2LY-0626-LY3499
Rating: 5 (1 reviews)

See all CHST3 antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.008 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

54.7 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

carbohydrate (chondroitin 6) sulfotransferase 3

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.

Biological Process

Carbohydrate metabolic process Source: UniProtKB-KW
Chondroitin sulfate biosynthetic process Source: UniProtKB
N-acetylglucosamine metabolic process Source: GO_Central
Sulfur compound metabolic process Source: UniProtKB

Cellular Location

Golgi apparatus membrane

Involvement in disease

Spondyloepiphyseal dysplasia with congenital joint dislocations (SEDCJD):
A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood.

Topology

Cytoplasmic: 1-20
Helical: 21-38
Lumenal: 39-479

Guan, Y., Sun, C., Zou, F., Wang, H., Lu, F., Song, J., ... & Ma, X. (2021). Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus. Journal of Cellular and Molecular Medicine.

Albuz, B., Çetin, G. O., Özhan, B., Sarikepe, B., Anlas, Ö., Öztürk, M., ... & Gündüz, C. N. S. (2020). A novel nonsense mutation in CHST3 in a Turkish patient with spondyloepiphyseal dysplasia, Omani type. Clinical dysmorphology, 29(1), 61-64.

Baidoe-Ansah, D., Sakib, M. S., Jia, S., Fischer, A., Kaushik, R., & Dityatev, A. (2019). Epigenetic mechanism of carbohydrate sulfotransferase 3 (CHST3) downregulation in the aging brain. bioRxiv, 741355.

Superti-Furga, A., & Unger, S. (2019). CHST3-related skeletal dysplasia.

Feng, L., Li, Y., Li, Y., Jiang, Y., Wang, N., Yuan, D., & Fan, J. (2018). Whole exome sequencing detects CHST3 mutation in patient with acute promyelocytic leukemia: a case report. Medicine, 97(36).

Wang, L., Guo, X., Yi, J., Qu, C., Lei, J., Guo, Q., & Han, J. (2018). The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats. Journal of Trace Elements in Medicine and Biology, 50, 123-129.

Yorifuji, K., Uemura, Y., Horibata, S., Tsuji, G., Suzuki, Y., Miyagawa, K., ... & Emoto, N. (2018). CHST3 and CHST13 polymorphisms as predictors of bosentan-induced liver toxicity in Japanese patients with pulmonary arterial hypertension. Pharmacological research, 135, 259-264.

Muys, J., Blaumeiser, B., Jacquemyn, Y., & Janssens, K. (2017). Prenatal homozygosity mapping detects a novel mutation in CHST3 in a fetus with skeletal dysplasia and joint dislocations. Clinical case reports, 5(4), 440.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human CHST3 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CHST3 protein, His Tag (CAT#: V2LY-0526-LY8112)

Related Products

Mouse Recombinant CHST3 protein, His Tag (CAT#: V2LY-0526-LY8112)

Mouse Anti-CHST3 Recombinant Antibody (CBFYC-1863) (CAT#: CBMAB-C1927-FY)

Mouse Anti-CHST3 Recombinant Antibody (C8546) (CAT#: CBMAB-C8546-LY)

Mouse Anti-CHST3 Recombinant Antibody (1C4) (CAT#: CBMAB-C3732-LY)

Mouse Anti-CHST3 Recombinant Antibody (CBLC159-LY) (CAT#: CBMAB-C10982-LY)

Mouse Anti-CHST3 Recombinant Antibody (1D3) (CAT#: CBMAB-A1621-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry