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Mouse Anti-CHST3 Recombinant Antibody (CBLC159-LY) (CBMAB-C10982-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBLC159-LY
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1-110 mapping at the N-terminus of C6ST-1 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
carbohydrate (chondroitin 6) sulfotransferase 3
Introduction
This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq, Mar 2009]
Entrez Gene ID
9469
UniProt ID
Q7LGC8
Alternative Names
Carbohydrate Sulfotransferase 3; Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 0; Carbohydrate (Chondroitin 6) Sulfotransferase 3; Chondroitin 6-O-Sulfotransferase 1; Chondroitin 6 Sulfotransferase 1; EC 2.8.2.17; C6ST-1;
Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.
Biological Process
Carbohydrate metabolic process Source: UniProtKB-KW
Chondroitin sulfate biosynthetic process Source: UniProtKB
N-acetylglucosamine metabolic process Source: GO_Central
Sulfur compound metabolic process Source: UniProtKB
Cellular Location
Golgi apparatus membrane
Involvement in disease
Spondyloepiphyseal dysplasia with congenital joint dislocations (SEDCJD):
A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood.
Topology
Cytoplasmic: 1-20
Helical: 21-38
Lumenal: 39-479
More Infomation

Guan, Y., Sun, C., Zou, F., Wang, H., Lu, F., Song, J., ... & Ma, X. (2021). Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus. Journal of Cellular and Molecular Medicine.

Albuz, B., Çetin, G. O., Özhan, B., Sarikepe, B., Anlas, Ö., Öztürk, M., ... & Gündüz, C. N. S. (2020). A novel nonsense mutation in CHST3 in a Turkish patient with spondyloepiphyseal dysplasia, Omani type. Clinical dysmorphology, 29(1), 61-64.

Baidoe-Ansah, D., Sakib, M. S., Jia, S., Fischer, A., Kaushik, R., & Dityatev, A. (2019). Epigenetic mechanism of carbohydrate sulfotransferase 3 (CHST3) downregulation in the aging brain. bioRxiv, 741355.

Superti-Furga, A., & Unger, S. (2019). CHST3-related skeletal dysplasia.

Feng, L., Li, Y., Li, Y., Jiang, Y., Wang, N., Yuan, D., & Fan, J. (2018). Whole exome sequencing detects CHST3 mutation in patient with acute promyelocytic leukemia: a case report. Medicine, 97(36).

Wang, L., Guo, X., Yi, J., Qu, C., Lei, J., Guo, Q., & Han, J. (2018). The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats. Journal of Trace Elements in Medicine and Biology, 50, 123-129.

Yorifuji, K., Uemura, Y., Horibata, S., Tsuji, G., Suzuki, Y., Miyagawa, K., ... & Emoto, N. (2018). CHST3 and CHST13 polymorphisms as predictors of bosentan-induced liver toxicity in Japanese patients with pulmonary arterial hypertension. Pharmacological research, 135, 259-264.

Muys, J., Blaumeiser, B., Jacquemyn, Y., & Janssens, K. (2017). Prenatal homozygosity mapping detects a novel mutation in CHST3 in a fetus with skeletal dysplasia and joint dislocations. Clinical case reports, 5(4), 440.

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For research use only. Not intended for any clinical use.

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