Mouse Recombinant EPHA2 proteinECD (V2LY-0526-LY8236)

Name: Mouse Recombinant EPHA2 proteinECD
SKU: V2LY-0526-LY8236
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Protein Construction

This product is Mouse Recombinant EPHA2 proteinECD consist of Amino Acid: 1-535 and predicts a molecular mass of 57.03 kDa.

Molecule Mass

57.03 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-535

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

EPH Receptor A2

Research Area

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Biological Process

Activation of GTPase activity Source: UniProtKB
Axial mesoderm formation Source: Ensembl
Axon guidance Source: GO_Central
Blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: Ensembl
Bone remodeling Source: UniProtKB
Branching involved in mammary gland duct morphogenesis Source: UniProtKB
cAMP metabolic process Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Cell chemotaxis Source: UniProtKB
Cell migration Source: UniProtKB
Cell motility Source: UniProtKB
Defense response to Gram-positive bacterium Source: Ensembl
Ephrin receptor signaling pathway Source: UniProtKB
Inflammatory response Source: Ensembl
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Keratinocyte differentiation Source: UniProtKB
Lens fiber cell morphogenesis Source: UniProtKB
Mammary gland epithelial cell proliferation Source: UniProtKB
Multicellular organism development Source: ProtInc
Negative regulation of angiogenesis Source: Ensembl
Negative regulation of chemokine production Source: Ensembl
Negative regulation of lymphangiogenesis Source: Ensembl
Negative regulation of protein kinase B signaling Source: UniProtKB
Neural tube development Source: Ensembl
Notochord cell development Source: Ensembl
Notochord formation Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Osteoclast differentiation Source: UniProtKB
Pericyte cell differentiation Source: Ensembl
Positive regulation of bicellular tight junction assembly Source: ARUK-UCL
Positive regulation of kinase activity Source: GO_Central
Positive regulation of protein localization to plasma membrane Source: UniProtKB
Post-anal tail morphogenesis Source: Ensembl
Protein kinase B signaling Source: UniProtKB
Protein localization to plasma membrane Source: ARUK-UCL
Regulation of angiogenesis Source: UniProtKB
Regulation of blood vessel endothelial cell migration Source: UniProtKB
Regulation of cell adhesion mediated by integrin Source: UniProtKB
Regulation of ERK1 and ERK2 cascade Source: UniProtKB
Regulation of lamellipodium assembly Source: UniProtKB
Response to growth factor Source: UniProtKB
Skeletal system development Source: Ensembl
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vasculogenesis Source: Ensembl

Cellular Location

Cell membrane; Ruffle membrane; Lamellipodium membrane; Focal adhesion. Present at regions of cell-cell contacts but also at the leading edge of migrating cells (PubMed:19573808, PubMed:20861311). Relocates from the plasma membrane to the cytoplasmic and perinuclear regions in cancer cells (PubMed:18794797).

Involvement in disease

Cataract 6, multiple types (CTRCT6):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. CTRCT6 includes posterior polar and age-related cortical cataracts, among others. Posterior polar cataract is a subcapsular opacity, usually disk-shaped, located at the back of the lens. Age-related cortical cataract is a developmental punctate opacity restricted to the cortex. The cataract is white or cerulean, increases in number with age, but rarely affects vision.

Topology

Extracellular: 24-537
Helical: 538-558
Cytoplasmic: 559-976

PTM

Autophosphorylates. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-930 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-897 by PKB; serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Phosphorylated at Ser-897 in response to TNF by RPS6KA1 and RPS6KA3; RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). Phosphorylated at Ser-897 by PKA; blocks cell retraction induced by EPHA2 kinase activity (PubMed:27385333). Dephosphorylated by ACP1.
Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation (By similarity).

Wallaschek, N., Reuter, S., Silkenat, S., Wolf, K., Niklas, C., Kayisoglu, Ö., ... & Bartfeld, S. (2021). Ephrin receptor A2, the epithelial receptor for Epstein-Barr virus entry, is not available for efficient infection in human gastric organoids. PLoS pathogens, 17(2), e1009210.

Wang, H., & Qiu, W. (2021). EPHA2, a promising therapeutic target for hepatocellular carcinoma. Molecular & Cellular Oncology, 8(3), 1910009.

Ieguchi, K., & Maru, Y. (2019). Roles of EphA1/A2 and ephrin‐A1 in cancer. Cancer science, 110(3), 841-848.

Chen, J., Sathiyamoorthy, K., Zhang, X., Schaller, S., Perez White, B. E., Jardetzky, T. S., & Longnecker, R. (2018). Ephrin receptor A2 is a functional entry receptor for Epstein–Barr virus. Nature microbiology, 3(2), 172-180.

Peng, Q., Chen, L., Wu, W., Wang, J., Zheng, X., Chen, Z., ... & Ma, J. (2018). EPH receptor A2 governs a feedback loop that activates Wnt/β-catenin signaling in gastric cancer. Cell death & disease, 9(12), 1-16.

Zhang, H., Li, Y., Wang, H. B., Zhang, A., Chen, M. L., Fang, Z. X., ... & Zeng, M. S. (2018). Ephrin receptor A2 is an epithelial cell receptor for Epstein–Barr virus entry. Nature microbiology, 3(2), 1-8.

Qazi, M. A., Vora, P., Venugopal, C., Adams, J., Singh, M., Hu, A., ... & Singh, S. K. (2018). Cotargeting Ephrin Receptor Tyrosine Kinases A2 and A3 in Cancer Stem Cells Reduces Growth of Recurrent GlioblastomaTargeting EphA2 and EphA3 in Recurrent GBM. Cancer Research, 78(17), 5023-5037.

Langlois, A. C., Marinach, C., Manzoni, G., & Silvie, O. (2018). Plasmodium sporozoites can invade hepatocytic cells independently of the Ephrin receptor A2. PLoS One, 13(7), e0200032.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EPHA2 protein, GST Tag (CAT#: V2LY-0526-LY3873)

Human Recombinant EPHA2 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY3874)

Human Recombinant EPHA2, Active protein, GST Tag (CAT#: V2LY-0526-LY3875)

Human Recombinant EPHA2 protein, hFc Tag (CAT#: V2LY-0526-LY3876)

Human Recombinant EPHA2 protein, hFc Tag-1 (CAT#: V2LY-0526-LY3877)

Human Recombinant EPHA2 protein, His Tag (CAT#: V2LY-0526-LY3878)

Mouse Recombinant EPHA2 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY8237)

Mouse Recombinant EPHA2 protein, His Tag (CAT#: V2LY-0526-LY8238)

Human EPHA2 ELISA Kit (CAT#: V2LY-0626-LY4548)

Mouse Epha2 ELISA Kit (CAT#: V2LY-0626-LY5844)

Antibody Pairs

EPHA2 Matched Antibody Pair (382) (CAT#: APMAB-382LY)

EPHA2 Matched Antibody Pair (383) (CAT#: APMAB-383LY)

EPHA2 Matched Antibody Pair (384) (CAT#: APMAB-384LY)

Related Products

Human Recombinant EPHA2 protein, His Tag (CAT#: V2LY-0526-LY3878)

Human Recombinant EPHA2 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY3874)

Rabbit Anti-EPHA2 Recombinant Antibody (169) (CAT#: CBMAB-1162-YC)

Mouse Recombinant EPHA2 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY8237)

Human Recombinant EPHA2 protein, GST Tag (CAT#: V2LY-0526-LY3873)

Rabbit Anti-EPHA2 Recombinant Antibody (EP4760) (CAT#: CBMAB-1158-YC)

EPHA2 Matched Antibody Pair (383) (CAT#: APMAB-383LY)

Human Recombinant EPHA2 protein, hFc Tag-1 (CAT#: V2LY-0526-LY3877)

Mouse Anti-EPHA2 Recombinant Antibody (CBFYE-0232) (CAT#: CBMAB-E0483-FY)

Human Recombinant EPHA2 protein, hFc Tag (CAT#: V2LY-0526-LY3876)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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