Mouse Recombinant FSTL3 protein, His Tag (V2LY-0526-LY8346)

Name: Mouse Recombinant FSTL3 protein, His Tag
SKU: V2LY-0526-LY8346
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant FSTL3 protein, His Tag consist of Amino Acid: 1-256 and predicts a molecular mass of 26 kDa.

Molecule Mass

26 kDa

Sequence

Amino Acid: 1-256

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

follistatin-like 3 (secreted glycoprotein)

Function

Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.

Biological Process

Adrenal gland development Source: Ensembl
Cell differentiation Source: GO_Central
Cellular response to metal ion Source: Ensembl
Hematopoietic progenitor cell differentiation Source: UniProtKB
Kidney development Source: Ensembl
Lung development Source: Ensembl
Male gonad development Source: Ensembl
Negative regulation of activin receptor signaling pathway Source: UniProtKB
Negative regulation of BMP signaling pathway Source: UniProtKB
Negative regulation of osteoclast differentiation Source: UniProtKB
Negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway Source: UniProtKB
Ossification Source: UniProtKB-KW
Positive regulation of cell-cell adhesion Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of BMP signaling pathway Source: GO_Central
Regulation of transcription by RNA polymerase II Source: UniProtKB
Spermatogenesis Source: Ensembl

Cellular Location

Isoform 1: Secreted
Isoform 2: Nucleus. Although alternative initiation has been demonstrated and resulted in different localization, the major source of nuclear FSTL3 appears not to depend on translation initiation at Met-27 according to.

Involvement in disease

A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1.

Li, X., Zhang, H., Ma, X., Wang, Y., Han, X., Yang, Y., ... & Bao, Y. (2023). FSTL3 is highly expressed in adipose tissue of individuals with overweight or obesity and is associated with inflammation. Obesity, 31(1), 171-183.

Li, C. H., Fang, C. Y., Chan, M. H., Chen, C. L., Chang, Y. C., & Hsiao, M. (2023). The cytoplasmic expression of FSTL3 correlates with colorectal cancer progression, metastasis status and prognosis. Journal of Cellular and Molecular Medicine.

Liu, Y. J., Li, J. P., Zhang, Y., Nie, M. J., Zhang, Y. H., Liu, S. L., & Zou, X. (2021). FSTL3 is a prognostic biomarker in gastric cancer and is correlated with M2 macrophage infiltration. OncoTargets and therapy, 14, 4099.

Sun, F., Sun, P., Yang, X., Hu, L., Gao, J., & Tian, T. (2021). Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3β/β-catenin signaling pathway. Aging (Albany NY), 13(18), 22528.

Brown, M. L., Lopez, A., Meyer, N., Richter, A., & Thompson, T. B. (2021). FSTL3-Neutralizing antibodies enhance glucose-responsive insulin secretion in dysfunctional male mouse and human islets. Endocrinology, 162(10).

He, J., Liu, Q., Yu, S., Lei, M., Liu, J., Di, R., ... & Chu, M. (2021). Expression and functional analysis of the Follistatin‐like 3 (FSTL3) gene in the sheep ovary during the oestrous cycle. Reproduction in Domestic Animals, 56(3), 427-436.

Li, J., Shen, J., Qin, L., Lu, D., & Ding, E. (2021). LBX2-AS1 activates FSTL3 by binding to transcription factor RARα to foster proliferation, migration, and invasion of thyroid cancer. Frontiers in Genetics, 2255.

Gao, L., Chen, X., Wang, Y., & Zhang, J. (2020). Up-regulation of FSTL3, regulated by lncRNA DSCAM-AS1/miR-122-5p Axis, promotes proliferation and migration of non-small cell lung Cancer cells. OncoTargets and therapy, 13, 2725.

Runhua, M., Qiang, J., Yunqing, S., Wenjun, D., & Chunsheng, W. (2019). FSTL3 induces lipid accumulation and inflammatory response in macrophages and associates with atherosclerosis. Journal of Cardiovascular Pharmacology, 74(6), 566-573.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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