Mouse Recombinant FZD1 protein, His Tag (V2LY-0526-LY8347)

Name: Mouse Recombinant FZD1 protein, His Tag
SKU: V2LY-0526-LY8347
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant FZD1 protein, His Tag consist of Amino Acid: 1-248 and predicts a molecular mass of 21 kDa.

Molecule Mass

21 kDa

Sequence

Amino Acid: 1-248

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Frizzled Class Receptor 1

Function

Receptor for Wnt proteins (PubMed:10557084).

Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (PubMed:10557084).

Contradictory results showing activation by WNT7B have been described for mouse (By similarity).

Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:10557084).

The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:10557084).

A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).

(Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium.

Biological Process

Autocrine signaling Source: BHF-UCL
Canonical Wnt signaling pathway Source: UniProtKB
Canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation Source: BHF-UCL
Canonical Wnt signaling pathway involved in osteoblast differentiation Source: BHF-UCL
Cell-cell signaling Source: BHF-UCL
Neuron differentiation Source: UniProtKB
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of neuron projection development Source: ARUK-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Presynapse assembly Source: ParkinsonsUK-UCL
Response to drug Source: BHF-UCL
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 73-322
Helical: 323-343
Cytoplasmic: 344-354
Helical: 355-375
Extracellular: 376-402
Helical: 403-423
Cytoplasmic: 424-445
Helical: 446-466
Extracellular: 467-489
Helical: 490-510
Cytoplasmic: 511-536
Helical: 537-557
Extracellular: 558-601
Helical: 602-622
Cytoplasmic: 623-647

PTM

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Jia, F., Zhang, L., Jiang, Z., Tan, G., & Wang, Z. (2023). FZD1/KLF10-hsa-miR-4762-5p/miR-224-3p-circular RNAs axis as prognostic biomarkers and therapeutic targets for glioblastoma: a comprehensive report. BMC Medical Genomics, 16(1), 21.

Liu, X., Low, S. K., Atkins, J. R., Wu, J. Q., Reay, W. R., Cairns, H. M., ... & Cairns, M. J. (2020). Wnt receptor gene FZD1 was associated with schizophrenia in genome-wide SNP analysis of the Australian Schizophrenia Research Bank cohort. Australian & New Zealand Journal of Psychiatry, 54(9), 902-908.

Peng, Q., Wang, L., Zhao, D., Lv, Y., Wang, H., Chen, G., ... & Xu, W. (2019). Overexpression of FZD1 is associated with a good prognosis and resistance of sunitinib in clear cell renal cell carcinoma. Journal of Cancer, 10(5), 1237.

Wang, Y. H., Imai, Y., Shiseki, M., Tanaka, J., & Motoji, T. (2018). Knockdown of the Wnt receptor Frizzled-1 (FZD1) reduces MDR1/P-glycoprotein expression in multidrug resistant leukemic cells and inhibits leukemic cell proliferation. Leukemia research, 67, 99-108.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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