Mouse Recombinant GAD2 protein (V2LY-0526-LY8348)

Name: Mouse Recombinant GAD2 protein
SKU: V2LY-0526-LY8348
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Mouse

Protein Construction

This product is Mouse Recombinant GAD2 protein consist of Amino Acid: 1-585 and predicts a molecular mass of 65.4 kDa.

Molecule Mass

65.4 kDa

Sequence

Amino Acid: 1-585

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Glutamate Decarboxylase 2

Function

Catalyzes the production of GABA.

Biological Process

Chemical synaptic transmission Source: ProtInc
Glutamate decarboxylation to succinate Source: ProtInc
Neurotransmitter biosynthetic process Source: UniProtKB-KW
Response to drug Source: Ensembl

Cellular Location

Cytosol; Presynaptic cell membrane; Golgi apparatus membrane; Cytoplasmic vesicle. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.

PTM

Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GAD2 protein, GST Tag (CAT#: V2LY-0526-LY4235)

Mouse Recombinant GAD2 protein, GST Tag (CAT#: V2LY-0526-LY8349)

GAD2 Matched Antibody Pair (460) (CAT#: APMAB-460LY)

Antibody Pairs

GAD2 Matched Antibody Pair (460) (CAT#: APMAB-460LY)

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Rabbit Anti-GAD2 Recombinant Antibody (CBLG1-481) (CAT#: V2LY-1225-LY585)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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