Mouse Recombinant KIRREL3 protein, His Tag (V2LY-0526-LY8592)

Name: Mouse Recombinant KIRREL3 protein, His Tag
SKU: V2LY-0526-LY8592
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant KIRREL3 protein, His Tag consist of Amino Acid: 1-535 and predicts a molecular mass of 57.5 kDa.

Molecule Mass

57.5 kDa

Sequence

Amino Acid: 1-535

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>96% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium Citrate, NaCl, Tween80, Trehalose

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

kin of IRRE like 3 (Drosophila)

Function

Synaptic adhesion molecule required for the formation of target-specific synapses. Required for formation of target-specific synapses at hippocampal mossy fiber synapses. Required for formation of mossy fiber filopodia, the synaptic structures connecting dentate granule and GABA neurons. Probably acts as a homophilic adhesion molecule that promotes trans-cellular interactions and stabilize mossy fiber filipodia contact and subsequent synapse formation. Required for the coalescence of vomeronasal sensory neuron axons. May be involved in the hematopoietic supportive capacity of stroma cells; the secreted extracellular domain is directly responsible for supporting hematopoietic stem cells.

Biological Process

Cell-cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Glomerulus morphogenesisIEA:Ensembl
HemopoiesisISS:UniProtKB
Hippocampus developmentISS:UniProtKB
Homophilic cell adhesion via plasma membrane adhesion moleculesISS:UniProtKB
Inter-male aggressive behaviorIEA:Ensembl
Neuron migrationIEA:Ensembl
Neuron projection morphogenesisIEA:Ensembl
Principal sensory nucleus of trigeminal nerve developmentIEA:Ensembl
Synapse assemblyISS:UniProtKB

Cellular Location

Cell membrane
Processed kin of IRRE-like protein 3: Secreted

Involvement in disease

A chromosomal aberration involving KIRREL3 and CDH15 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).

Topology

Extracellular: 22-535
Helical: 536-556
Cytoplasmic: 557-778

PTM

Undergoes proteolysis by a metalloprotease and gives rise to a soluble form.

Querzani, A., Sirchia, F., Rustioni, G., Rossi, A., Orsini, A., Marseglia, G. L., ... & Foiadelli, T. (2023). KIRREL3-related disorders: a case report confirming the radiological features and expanding the clinical spectrum to a less severe phenotype. Italian Journal of Pediatrics, 49(1), 99.

Traenkner, D., Shennib, O., Johnson, A., Weinbrom, A., Taylor, M. R., & Williams, M. E. (2023). Modular Splicing is Linked to Evolution in the Synapse-Specificity Molecule Kirrel3. eNeuro.

Yang, B., Zhang, X., Zhou, H., Zhang, X., Yang, W., Lu, J., ... & Tian, F. (2022). Preliminary study on the role and mechanism of KIRREL3 in the development of esophageal squamous cell carcinoma. Pathology-Research and Practice, 237, 154025.

Ciaccio, C., Leonardi, E., Polli, R., Murgia, A., D'Arrigo, S., Granocchio, E., ... & Esposito, S. (2021). A missense de novo variant in the CASK-interactor KIRREL3 gene leading to neurodevelopmental disorder with mild cerebellar hypoplasia. Neuropediatrics, 52(06), 484-488.

Taylor, M. R., Martin, E. A., Sinnen, B., Trilokekar, R., Ranza, E., Antonarakis, S. E., & Williams, M. E. (2020). Kirrel3-mediated synapse formation is attenuated by disease-associated missense variants. Journal of Neuroscience, 40(28), 5376-5388.

Völker, L. A., Maar, B. A., Pulido Guevara, B. A., Bilkei‐Gorzo, A., Zimmer, A., Brönneke, H., ... & Hoehne, M. (2018). Neph2/Kirrel3 regulates sensory input, motor coordination, and home‐cage activity in rodents. Genes, Brain and Behavior, 17(8), e12516.

Hisaoka, T., Komori, T., Kitamura, T., & Morikawa, Y. (2018). Abnormal behaviours relevant to neurodevelopmental disorders in Kirrel3-knockout mice. Scientific Reports, 8(1), 1408.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Recombinant KIRREL3 protein, His Tag (CAT#: V2LY-0526-LY9502)

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Rat Recombinant KIRREL3 protein, His Tag (CAT#: V2LY-0526-LY9502)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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