Mouse Recombinant NEGR1 protein, His Tag (V2LY-0526-LY8694)

Name: Mouse Recombinant NEGR1 protein, His Tag
SKU: V2LY-0526-LY8694
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant NEGR1 protein, His Tag consist of Amino Acid: 1-318 and predicts a molecular mass of 33 kDa.

Molecule Mass

33 kDa

Sequence

Amino Acid: 1-318

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>94% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, PMSF

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

neuronal growth regulator 1

Function

May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain (By similarity).

Biological Process

Brain development Source: Ensembl
Cell-cell adhesion Source: Ensembl
Feeding behavior Source: Ensembl
Locomotory behavior Source: Ensembl
Positive regulation of neuron projection development Source: Ensembl
Regulation of synapse assembly Source: Ensembl

Cellular Location

Cell membrane

Pischedda, F., Ghirelli, A., Tripathi, V., & Piccoli, G. (2023). Negr1-Derived Peptides Trigger ALK Degradation and Halt Neuroblastoma Progression In Vitro and In Vivo. Pharmaceutics, 15(9), 2307.

Qiu, L., He, J., Chen, H., Xu, X., & Tao, Y. (2022). CircDLGAP4 overexpression relieves oxygen-glucose deprivation-induced neuronal injury by elevating NEGR1 through sponging miR-503-3p. Journal of Molecular Histology, 53(2), 321-332.

Kaare, M., Mikheim, K., Lilleväli, K., Kilk, K., Jagomäe, T., Leidmaa, E., ... & Philips, M. A. (2021). High-fat diet induces pre-diabetes and distinct sex-specific metabolic alterations in negr1-deficient mice. Biomedicines, 9(9), 1148.

Yan, Y., Chen, L., Zhou, J., & Xie, L. (2020). SNHG12 inhibits oxygen‑glucose deprivation‑induced neuronal apoptosis via the miR‑181a‑5p/NEGR1 axis. Molecular Medicine Reports, 22(5), 3886-3894.

Wang, X., Cheng, W., Zhu, J., Yin, H., Chang, S., Yue, W., & Yu, H. (2020). Integrating genome-wide association study and expression quantitative trait loci data identifies NEGR1 as a causal risk gene of major depression disorder. Journal of affective disorders, 265, 679-686.

Dong, Y., Fan, X., Wang, Z., Zhang, L., & Guo, S. (2020). Circ_HECW2 functions as a miR-30e-5p sponge to regulate LPS-induced endothelial-mesenchymal transition by mediating NEGR1 expression. Brain Research, 1748, 147114.

Carboni, L., Pischedda, F., Piccoli, G., Lauria, M., Musazzi, L., Popoli, M., ... & Domenici, E. (2020). Depression-associated gene Negr1-Fgfr2 pathway is altered by antidepressant treatment. Cells, 9(8), 1818.

Breton, E., Gagné-Ouellet, V., Thibeault, K., Guérin, R., Van Lieshout, R., Perron, P., ... & Bouchard, L. (2020). Placental NEGR1 DNA methylation is associated with BMI and neurodevelopment in preschool-age children. Epigenetics, 15(3), 323-335.

Singh, K., Jayaram, M., Kaare, M., Leidmaa, E., Jagomäe, T., Heinla, I., ... & Vasar, E. (2019). Neural cell adhesion molecule Negr1 deficiency in mouse results in structural brain endophenotypes and behavioral deviations related to psychiatric disorders. Scientific reports, 9(1), 5457.

Noh, K., Lee, H., Choi, T. Y., Joo, Y., Kim, S. J., Kim, H., ... & Lee, S. J. (2019). Negr1 controls adult hippocampal neurogenesis and affective behaviors. Molecular psychiatry, 24(8), 1189-1205.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NEGR1 protein, His Tag (CAT#: V2LY-0526-LY5731)

Related Products

Human Recombinant NEGR1 protein, His Tag (CAT#: V2LY-0526-LY5731)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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