Mouse Recombinant PDI protein, His Tag (V2LY-0526-LY8806)

Name: Mouse Recombinant PDI protein, His Tag
SKU: V2LY-0526-LY8806
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant PDI protein, His Tag consist of Amino Acid: 1-506 and predicts a molecular mass of 56.2 kDa.

Molecule Mass

56.2 kDa

Sequence

Amino Acid: 1-506

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Prolyl 4-Hydroxylase Subunit Beta

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).

Biological Process

Cellular response to hypoxiaManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to interleukin-7IEA:Ensembl
Interleukin-12-mediated signaling pathwayTAS:Reactome
Interleukin-23-mediated signaling pathwayTAS:Reactome
Peptidyl-proline hydroxylation to 4-hydroxy-L-prolineManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell adhesionManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of viral entry into host cellManual Assertion Based On ExperimentIMP:UniProtKB
Protein foldingManual Assertion Based On ExperimentIBA:GO_Central
Protein folding in endoplasmic reticulumManual Assertion Based On ExperimentIDA:FlyBase
Regulation of oxidative stress-induced intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Response to endoplasmic reticulum stressManual Assertion Based On ExperimentIMP:BHF-UCL

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum lumen
Melanosome
Cell membrane
Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces (PubMed:11181151).
Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:10636893).
Colocalizes with MTTP in the endoplasmic reticulum (PubMed:23475612).

Involvement in disease

Cole-Carpenter syndrome 1 (CLCRP1):
A form of Cole-Carpenter syndrome, a disorder characterized by features of osteogenesis imperfecta such as bone deformities and severe bone fragility with frequent fractures, in association with craniosynostosis, ocular proptosis, hydrocephalus, growth failure and distinctive facial features. Craniofacial findings include marked frontal bossing, midface hypoplasia, and micrognathia. Despite the craniosynostosis and hydrocephalus, intellectual development is normal. CLCRP1 inheritance is autosomal dominant.

Feng, D., Li, L., Li, D., Wu, R., Zhu, W., Wang, J., ... & Han, P. (2023). Prolyl 4-hydroxylase subunit beta (P4HB) could serve as a prognostic and radiosensitivity biomarker for prostate cancer patients. European Journal of Medical Research, 28(1), 245.

Wang, F., Yu, Z., Liu, X., Hu, F., Liu, X., Fu, X., ... & Zou, Z. (2023). A meta-analysis and bioinformatics analysis of p4hb expression levels in the prognosis of cancer patients. Pathology-Research and Practice, 154474.

Bai, F., Yu, K., Yang, Y., Zhang, Y., Ding, L., An, X., ... & Yang, X. (2022). Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy. Frontiers in Genetics, 13, 965816.

Wu, Y., Peng, Y., Guan, B., He, A., Yang, K., He, S., ... & Zhou, L. (2021). P4HB: A novel diagnostic and prognostic biomarker for bladder carcinoma. Oncology letters, 21(2), 1-1.

Yang, W., Wu, X., & Zhou, F. (2021). Collagen type X alpha 1 (COL10A1) contributes to cell proliferation, migration, and invasion by targeting prolyl 4-hydroxylase beta polypeptide (P4HB) in breast cancer. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 27, e928919-1.

Zhang, J., Guo, S., Wu, Y., Zheng, Z. C., Wang, Y., & Zhao, Y. (2019). P4HB, a novel hypoxia target gene related to gastric cancer invasion and metastasis. BioMed research international, 2019.

Zhu, Z., He, A., Lv, T., Xu, C., Lin, L., & Lin, J. (2019). Overexpression of P4HB is correlated with poor prognosis in human clear cell renal cell carcinoma. Cancer Biomarkers, 26(4), 431-439.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human P4HB ELISA Kit (CAT#: V2LY-0626-LY1227)

Cattle P4HB ELISA Kit (CAT#: V2LY-0626-LY2629)

Mouse P4hb ELISA Kit (CAT#: V2LY-0626-LY2916)

Human Recombinant P4HB protein, His Tag (CAT#: V2LY-0526-LY6346)

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Isotype control

For research use only. Not intended for any clinical use.

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