Mouse Recombinant RAB2A protein, His Tag (V2LY-0526-LY8820)

Name: Mouse Recombinant RAB2A protein, His Tag
SKU: V2LY-0526-LY8820
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant RAB2A protein, His Tag consist of Amino Acid: 1-212 and predicts a molecular mass of 25.8 kDa.

Molecule Mass

25.8 kDa

Sequence

Amino Acid: 1-212

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

RAB2A, Member RAS Oncogene Family

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi.

Biological Process

Biological Process endoplasmic reticulum to Golgi vesicle-mediated transportManual Assertion Based On ExperimentTAS:ProtInc
Biological Process Golgi organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein transportIEA:UniProtKB-KW
Biological Process vesicle-mediated transportManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Endoplasmic reticulum-Golgi intermediate compartment membrane
Melanosome
Endoplasmic reticulum membrane
Golgi apparatus membrane
Cytoplasmic vesicle, secretory vesicle, acrosome
Localized in the Golgi apparatus in the round spermatids and in the acrosome in the elongating spermatid (By similarity).
Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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