Mouse Recombinant RSPO1 protein, His Tag (V2LY-0526-LY8850)

Name: Mouse Recombinant RSPO1 protein, His Tag
SKU: V2LY-0526-LY8850
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

CHO Stable Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant RSPO1 protein, His Tag consist of Amino Acid: 1-265 and predicts a molecular mass of 28.5 kDa.

Molecule Mass

28.5 kDa

Sequence

Amino Acid: 1-265

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

R-Spondin 1

Function

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720).
Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805).

Biological Process

Biological Process positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of receptor internalizationManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process Wnt signaling pathwayIEA:UniProtKB-KW

Cellular Location

Secreted
Nucleus
Seems to mainly localize to nucleoli.

Involvement in disease

Keratoderma, palmoplantar, with squamous cell carcinoma of skin and sex reversal (PKKSCC):
A recessive syndrome characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant RSPO1 protein, His Tag (CAT#: V2LY-0526-LY6623)

Human Recombinant RSPO1 protein, His Tag-1 (CAT#: V2LY-0526-LY6624)

Human Recombinant RSPO1 protein (CAT#: V2LY-0526-LY6625)

Mouse Rspo1 ELISA Kit (CAT#: V2LY-0626-LY3003)

Human RSPO1 ELISA Kit (CAT#: V2LY-0626-LY5197)

Related Products

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Mouse Anti-RSPO1 Recombinant Antibody (CBT1079) (CAT#: V2LY-0625-LY3050)

Mouse Anti-RSPO1 Recombinant Antibody (7A6) (CAT#: CBMAB-R3743-CN)

Mouse Anti-RSPO1 Recombinant Antibody (1C3) (CAT#: CBMAB-R3739-CN)

Mouse Anti-RSPO1 Recombinant Antibody (2F10) (CAT#: CBMAB-R3741-CN)

Human Recombinant RSPO1 protein, His Tag (CAT#: V2LY-0526-LY6623)

Human RSPO1 ELISA Kit (CAT#: V2LY-0626-LY5197)

Mouse Anti-RSPO1 Recombinant Antibody (CBT4135) (CAT#: V2LY-0625-LY2252)

Rat Anti-RSPO1 Recombinant Antibody (15H132) (CAT#: CBMAB-R3738-CN)

Mouse Anti-RSPO1 Recombinant Antibody (422407) (CAT#: CBMAB-R3742-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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