Mouse Recombinant SIGLEC10 protein, ECD, hFc Tag (V2LY-0526-LY8901)

Name: Mouse Recombinant SIGLEC10 protein, ECD, hFc Tag
SKU: V2LY-0526-LY8901
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

hFc Tag

Protein Construction

This product is Mouse Recombinant SIGLEC10 protein, ECD, hFc Tag consist of Amino Acid: 1-543 and predicts a molecular mass of 85.59 kDa.

Molecule Mass

85.59 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-543

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

sialic acid binding Ig like lectin 10

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid (By similarity).
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, seems to act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules (PubMed:11284738, PubMed:12163025).
Involved in negative regulation of B-cell antigen receptor signaling. The inhibition of B cell activation is dependent on PTPN6/SHP-1 (By similarity).
In association with CD24 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90 (By similarity).
In association with CD24 may regulate the immune repsonse of natural killer (NK) cells (PubMed:25450598).
Plays a role in the control of autoimmunity (By similarity).
During initiation of adaptive immune responses by CD8-alpha+ dendritic cells inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes. The function seems to implicate recruitment of PTPN6/SHP-1, which dephosphorylates NCF1 of the NADPH oxidase complex consequently promoting phagosomal acidification (By similarity).

Biological Process

Biological Process adaptive immune responseIEA:UniProtKB-KW
Biological Process cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process innate immune responseIEA:UniProtKB-KW
Biological Process negative regulation of inflammatory response to woundingManual Assertion Based On ExperimentIPI:UniProtKB

Cellular Location

Isoform 1
Cell membrane
Isoform 2
Cell membrane
Isoform 3
Cell membrane
Isoform 4
Cell membrane
Isoform 5
Secreted

Topology

Extracellular: 17-550
Helical: 551-571
Cytoplasmic: 572-697

PTM

Phosphorylation of Tyr-667 is involved in binding to PTPN6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SIGLEC10 protein, ECD, His Tag (CAT#: V2LY-0526-LY6771)

Human Recombinant SIGLEC10 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY6772)

Related Products

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Human Recombinant SIGLEC10 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY6772)

Human Recombinant SIGLEC10 protein, ECD, His Tag (CAT#: V2LY-0526-LY6771)

Mouse Anti-SIGLEC10 Recombinant Antibody (CBXS-2674) (CAT#: CBMAB-S5426-CQ)

Mouse Anti-SIGLEC10 Recombinant Antibody (CBYC-P839) (CAT#: CBMAB-P3675-YC)

Mouse Anti-SIGLEC10 Recombinant Antibody (CBXS-5183) (CAT#: CBMAB-S0274-CQ)

Rabbit Anti-SIGLEC10 Recombinant Antibody (D4E3Z) (CAT#: CBMAB-CP2434-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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