Pig IFNAR1 ELISA Kit (V2LY-0626-LY983)

Name: Pig IFNAR1 ELISA Kit
SKU: V2LY-0626-LY983
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.055 ng/mL

Detection Range

0.1-40 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Pig

Assay Type

Sandwich

Reactivity

Pig

Assay Time

1.5 h

Molecule Mass

63.2 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Interferon Alpha And Beta Receptor Subunit 1

Alternative Names

Interferon Alpha And Beta Receptor Subunit 1

Function

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986).

Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986).

Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (PubMed:7665574, PubMed:21854986, PubMed:32972995).

Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).

Biological Process

Cellular response to interferon-alpha Source: UniProtKB
Cytokine-mediated signaling pathway Source: GO_Central
Positive regulation of cellular respiration Source: ARUK-UCL
Receptor signaling pathway via JAK-STAT Source: ProtInc
Response to lipopolysaccharide Source: UniProtKB
Response to virus Source: ProtInc
Type I interferon signaling pathway Source: UniProtKB

Cellular Location

Isoform 1: Lysosome; Cell membrane; Late endosome. Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes.

Topology

Extracellular: 28-436
Helical: 437-457
Cytoplasmic: 458-557

PTM

Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985).
Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985). Phosphorylated on tyrosine residues by TYK2 tyrosine kinase (PubMed:7526154). Phosphorylated on tyrosine residues in response to interferon (PubMed:10049744).
Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.

Abolhassani, H., Landegren, N., Bastard, P., Materna, M., Modaresi, M., Du, L., ... & Pan-Hammarström, Q. (2022). Inherited IFNAR1 deficiency in a child with both critical COVID-19 pneumonia and multisystem inflammatory syndrome. Journal of Clinical Immunology, 42(3), 471-483.

Khanmohammadi, S., Rezaei, N., Khazaei, M., & Shirkani, A. (2022). A case of autosomal recessive interferon alpha/beta receptor alpha chain (IFNAR1) deficiency with severe COVID-19. Journal of clinical immunology, 1-6.

Bastard, P., Hsiao, K. C., Zhang, Q., Choin, J., Best, E., Chen, J., ... & Casanova, J. L. (2022). A loss-of-function IFNAR1 allele in Polynesia underlies severe viral diseases in homozygotes. Journal of Experimental Medicine, 219(6), e20220028.

Bastard, P., Manry, J., Chen, J., Rosain, J., Seeleuthner, Y., AbuZaitun, O., ... & Zhang, S. Y. (2021). Herpes simplex encephalitis in a patient with a distinctive form of inherited IFNAR1 deficiency. The Journal of clinical investigation, 131(1).

Xu, F., Wang, H., Tian, J., & Xu, H. (2021). Down-regulation of ID2-AS1 alleviates the neuronal injury induced by 1-methy1-4-phenylpyridinium in human neuroblastoma cell line SH-SY5Y cells through regulating miR-199a-5p/IFNAR1/JAK2/STAT1 Axis. Neurochemical Research, 46, 2192-2203.

Shemesh, M., Lochte, S., Piehler, J., & Schreiber, G. (2021). IFNAR1 and IFNAR2 play distinct roles in initiating type I interferon–induced JAK-STAT signaling and activating STATs. Science signaling, 14(710), eabe4627.

Saminathan, M., Singh, K. P., Vineetha, S., Maity, M., Biswas, S. K., Manjunathareddy, G. B., ... & Mertens, P. P. C. (2020). Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid gestation. Scientific Reports, 10(1), 2164.

Tian, J., Kang, H., Huang, J., Li, Z., Pan, Y., Li, Y., ... & Qu, L. (2020). Feline calicivirus strain 2280 p30 antagonizes type I interferon-mediated antiviral innate immunity through directly degrading IFNAR1 mRNA. PLoS Pathogens, 16(10), e1008944.

Hernandez, N., Bucciol, G., Moens, L., Le Pen, J., Shahrooei, M., Goudouris, E., ... & Bossuyt, X. (2019). Inherited IFNAR1 deficiency in otherwise healthy patients with adverse reaction to measles and yellow fever live vaccines. Journal of Experimental Medicine, 216(9), 2057-2070.

Zhang, G., Deweerd, N. A., Stifter, S. A., Liu, L., Zhou, B., Wang, W., ... & Feng, C. G. (2018). A proline deletion in IFNAR1 impairs IFN-signaling and underlies increased resistance to tuberculosis in humans. Nature communications, 9(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IFNAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY5043)

Human Recombinant IFNAR1 protein, hFc Tag (CAT#: V2LY-0526-LY5044)

Human Recombinant IFNAR1 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5045)

Mouse Recombinant IFNAR1 protein, His Tag (CAT#: V2LY-0526-LY8567)

Rabbit Recombinant IFNAR1 protein, His Tag (CAT#: V2LY-0526-LY9137)

Rat Recombinant IFNAR1 protein, His Tag (CAT#: V2LY-0526-LY9494)

Rhesus Recombinant IFNAR1 protein (CAT#: V2LY-0526-LY9760)

Rhesus Recombinant IFNAR1 protein, hFc Tag (CAT#: V2LY-0526-LY9761)

Rhesus Recombinant IFNAR1 protein, His Tag (CAT#: V2LY-0526-LY9762)

IFNAR1 Matched Antibody Pair (604) (CAT#: APMAB-604LY)

Antibody Pairs

IFNAR1 Matched Antibody Pair (604) (CAT#: APMAB-604LY)

Related Products

Rabbit Anti-IFNAR1 Recombinant Antibody (CBNH-077) (CAT#: CBMAB-R4402-CN)

Human Recombinant IFNAR1 protein, hFc Tag (CAT#: V2LY-0526-LY5044)

Human Recombinant IFNAR1 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5045)

Mouse Anti-IFNAR1 Recombinant Antibody (MAR1-5A3) (CAT#: CBMAB-1022-LY)

IFNAR1 Matched Antibody Pair (604) (CAT#: APMAB-604LY)

Mouse Anti-IFNAR1 Recombinant Antibody (A388) (CAT#: CBMAB-AP10816LY)

Mouse Anti-IFNAR1 Recombinant Antibody (AG91) (CAT#: V2LY-0524-LY137)

Mouse Recombinant IFNAR1 protein, His Tag (CAT#: V2LY-0526-LY8567)

Rhesus Recombinant IFNAR1 protein, His Tag (CAT#: V2LY-0526-LY9762)

Rhesus Recombinant IFNAR1 protein, hFc Tag (CAT#: V2LY-0526-LY9761)

Isotype control

For research use only. Not intended for any clinical use.

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