Cattle APOH ELISA Kit (V2LY-0626-LY327)

Name: Cattle APOH ELISA Kit
SKU: V2LY-0626-LY327
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.11 μg/mL

Detection Range

0.2-70 μg/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Cattle

Assay Type

Sandwich

Reactivity

Cattle

Assay Time

1.5 h

Molecule Mass

38.3 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Apolipoprotein H

Function

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Biological Process

Blood coagulation, intrinsic pathway Source: BHF-UCL
Negative regulation of angiogenesis Source: BHF-UCL
Negative regulation of blood coagulation Source: BHF-UCL
Negative regulation of endothelial cell migration Source: BHF-UCL
Negative regulation of endothelial cell proliferation Source: BHF-UCL
Negative regulation of fibrinolysis Source: BHF-UCL
Negative regulation of myeloid cell apoptotic process Source: BHF-UCL
Negative regulation of smooth muscle cell apoptotic process Source: BHF-UCL
Plasminogen activation Source: BHF-UCL
Platelet degranulation Source: Reactome
Positive regulation of blood coagulation Source: BHF-UCL
Positive regulation of lipoprotein lipase activity Source: BHF-UCL
Regulation of fibrinolysis Source: BHF-UCL
Triglyceride metabolic process Source: BHF-UCL
Triglyceride transport Source: BHF-UCL

Cellular Location

Secreted

PTM

N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.

Liu, Y., Maiers, J. L., Rui, Y., Jiang, X., Guleng, B., & Ren, J. (2021). Apolipoprotein H drives hepatitis B surface antigen retention and endoplasmic reticulum stress during hepatitis B virus infection. The International Journal of Biochemistry & Cell Biology, 131, 105906.

Liu, Y., Maiers, J. L., Wu, Y., Fu, B., Guo, X., Huang, X., ... & Ren, J. (2021). 713d Downregulation of Apolipoprotein H Induce Hepatocyte Steatosis to Promote Gut Microbiota Dysbiosis in Chronic Hepatitis B Infection. Gastroenterology, 161(2), e26.

Tan, Y., Bian, Y., Song, Y., Zhang, Q., & Wan, X. (2021). Exosome-Contained APOH Associated With Antiphospholipid Syndrome. Frontiers in Immunology, 12, 1237.

Wajda, A., Sowińska, A., Haładyj, E., Stypińska, B., Nałęcz-Janik, J., Jagodziński, P. P., ... & Paradowska-Gorycka, A. (2020). Tissue factor and human apolipoprotein H genetic variants and pro-inflammatory cytokines in systemic lupus erythematosus patients. Clinical and Experimental Rheumatology.

Khatri, S., Mellins, E. D., Torok, K. S., Bukhari, S. A., & Astakhova, K. (2020). Combined assay for detecting autoantibodies to nucleic acids and apolipoprotein H in patients with systemic lupus erythematosus. In Nucleic Acid Detection and Structural Investigations (pp. 57-71). Humana, New York, NY.

Abi Jomaa, R. (2020). Effects of Apolipoprotein H and STAT3 on Cell Clustering of Glioblastoma Multiforme Sub-Clones (Doctoral dissertation).

Zhang, X. P., Zeng, W., Liu, H., Tang, L., Wang, Q. Y., Cheng, Z. P., ... & Hu, Y. (2018). Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene. Current medical science, 38(2), 379-385.

e Cunha, C. S., Nyboer, B., Heiss, K., Sanches-Vaz, M., Fontinha, D., Wiedtke, E., ... & Mueller, A. K. (2017). Plasmodium berghei EXP-1 interacts with host Apolipoprotein H during Plasmodium liver-stage development. Proceedings of the National Academy of Sciences, 114(7), E1138-E1147.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant APOH protein, Domain V, His Tag (CAT#: V2LY-0526-LY2150)

Human Recombinant APOH protein, His Tag (CAT#: V2LY-0526-LY2151)

Human Recombinant APOH protein, His Tag-1 (CAT#: V2LY-0526-LY2152)

Rat Recombinant APOH protein, His Tag (CAT#: V2LY-0526-LY9162)

Rat Recombinant APOH protein, His Tag-1 (CAT#: V2LY-0526-LY9163)

Related Products

Human Recombinant APOH protein, His Tag (CAT#: V2LY-0526-LY2151)

Rabbit Anti-APOH Recombinant Antibody (CBYC-A699) (CAT#: CBMAB-A3253-YC)

Mouse Anti-APOH Recombinant Antibody (A3248) (CAT#: CBMAB-A3248-YC)

Human Recombinant APOH protein, Domain V, His Tag (CAT#: V2LY-0526-LY2150)

Mouse Anti-APOH Recombinant Antibody (H219) (CAT#: CBMAB-A3251-YC)

Human Recombinant APOH protein, His Tag-1 (CAT#: V2LY-0526-LY2152)

Rat Recombinant APOH protein, His Tag (CAT#: V2LY-0526-LY9162)

Mouse Anti-APOH Recombinant Antibody (CBYC-A700) (CAT#: CBMAB-A3254-YC)

Mouse Anti-APOH Recombinant Antibody (C2) (CAT#: CBMAB-A3245-YC)

Mouse Anti-APOH Recombinant Antibody (3F10) (CAT#: CBMAB-A3246-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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