Rat Ccl7 ELISA Kit (V2LY-0626-LY543)

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Tested Data
Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0015 ng/mL
Detection Range
0.003-0.9 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
10.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
C-C Motif Chemokine Ligand 7
Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Biological Process
Cell-cell signaling Source: ProtInc
Cellular calcium ion homeostasis Source: ProtInc
Cellular response to ethanol Source: Ensembl
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: ProtInc
Cytoskeleton organization Source: UniProtKB
Eosinophil chemotaxis Source: UniProtKB
G protein-coupled receptor signaling pathway Source: GO_Central
Inflammatory response Source: GO_Central
Lymphocyte chemotaxis Source: GO_Central
Monocyte chemotaxis Source: GO_Central
Neutrophil chemotaxis Source: GO_Central
Positive regulation of cell migration Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Positive regulation of natural killer cell chemotaxis Source: UniProtKB
Regulation of cell shape Source: UniProtKB
Response to gamma radiation Source: Ensembl
Signal transduction Source: ProtInc
Cellular Location
Secreted
PTM
O-glycosylated.

Waśkiel-Burnat, A., Niemczyk, A., Blicharz, L., Chmielińska, P., Zaremba, M., Gąsecka, A., ... & Rudnicka, L. (2021). Chemokine CC Motif Ligand 7 (CCL7), a Biomarker of Atherosclerosis, Is Associated with the Severity of Alopecia Areata: A Preliminary Study. Journal of Clinical Medicine, 10(22), 5418.

Jeong, M., Wang, Y. Y., Choi, J. Y., Lim, M. C., & Choi, J. H. (2021). CC Chemokine Ligand 7 Derived from Cancer-Stimulated Macrophages Promotes Ovarian Cancer Cell Invasion. Cancers, 13(11), 2745.

Niu, M., Luo, Z., Gong, S., Win, S., Kaplowitz, N., Jiang, Y., & Chen, P. (2020). Intestinal epithelial chemokine (CC motif) ligand 7 overexpression enhances acetaminophen-induced hepatotoxicity in mice. The American journal of pathology, 190(1), 57-67.

Chen, J., Dang, Y., Feng, W., Qiao, C., Liu, D., Zhang, T., ... & Xia, L. (2020). SOX18 promotes gastric cancer metastasis through transactivating MCAM and CCL7. Oncogene, 39(33), 5536-5552.

Kurzejamska, E., Sacharczuk, M., Landázuri, N., Kovtonyuk, O., Lazarczyk, M., Ananthaseshan, S., ... & Religa, P. (2019). Effect of chemokine (CC motif) ligand 7 (CCL7) and its receptor (CCR2) expression on colorectal Cancer behaviors. International journal of molecular sciences, 20(3), 686.

Mariya, S. S., Dewi, F. N., Villiandra, V., Paramastri, Y. A., Iskandriati, D., Saepuloh, U., ... & Sajuthi, D. (2019). Isolation and characterization of CC chemokine ligand 7 (CCL7) in cynomolgus macaques. HAYATI Journal of Biosciences, 26(3), 129-129.

Wu, Z., Tian, Y., Yu, Q., Li, H., Tian, Z., Jiang, H., ... & Yang, X. (2018). The expression and correlation between chemokine CCL7 and ABCE1 in non‑small cell lung cancer. Experimental and therapeutic medicine, 16(4), 3004-3010.

Liu, Y., Cai, Y., Liu, L., Wu, Y., & Xiong, X. (2018). Crucial biological functions of CCL7 in cancer. PeerJ, 6, e4928.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CCL7 protein, His Tag (CAT#: V2LY-0526-LY5481)

Mouse Recombinant CCL7 protein, His Tag (CAT#: V2LY-0526-LY8649)

Human CCL7 ELISA Kit (CAT#: V2LY-0626-LY426)

Human CCL7 ELISA Kit (2) (CAT#: V2LY-0626-LY4375)

Mouse Ccl7 ELISA Kit (CAT#: V2LY-0626-LY974)

Related Products

Mouse Ccl7 ELISA Kit (CAT#: V2LY-0626-LY974)

Human Recombinant CCL7 protein, His Tag (CAT#: V2LY-0526-LY5481)

Mouse Anti-CCL7 Recombinant Antibody (CBFYM-0950) (CAT#: CBMAB-M1099-FY)

Mouse Anti-CCL7 Recombinant Antibody (CBFYM-0951) (CAT#: CBMAB-M1100-FY)

Mouse Anti-CCL7 Recombinant Antibody (1015) (CAT#: CBMAB-C2032-LY)

Rabbit Anti-CCL7 Recombinant Antibody (CBFYM-0954) (CAT#: CBMAB-M1103-FY)

Human CCL7 ELISA Kit (CAT#: V2LY-0626-LY426)

Mouse Anti-CCL7 Recombinant Antibody (CBFYM-0071) (CAT#: CBMAB-M0080-FY)

Mouse Anti-CCL7 Recombinant Antibody (CBFYM-0947) (CAT#: CBMAB-M1096-FY)

Mouse Anti-CCL7 Recombinant Antibody (CBFYM-0948) (CAT#: CBMAB-M1097-FY)

Isotype control

For research use only. Not intended for any clinical use.

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