Rat Recombinant ACVR1B protein, hFc Tag (V2LY-0526-LY9158)

Name: Rat Recombinant ACVR1B protein, hFc Tag
SKU: V2LY-0526-LY9158
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

hFc Tag

Protein Construction

This product is Rat Recombinant ACVR1B protein, hFc Tag consist of Amino Acid: 1-126 and predicts a molecular mass of 38.4 kDa.

Molecule Mass

38.4 kDa

Sequence

Amino Acid: 1-126

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Activin A Receptor Type 1B

Function

Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.

Biological Process

Activin receptor signaling pathway
Cellular response to growth factor stimulus
Central nervous system development
Development of primary female sexual characteristics
Extrinsic apoptotic signaling pathway
G1/S transition of mitotic cell cycle
Hair follicle development
In utero embryonic development
Negative regulation of cell growth
Negative regulation of gene expression
Nervous system development
Nodal signaling pathway
Peptidyl-threonine phosphorylation
Positive regulation of activin receptor signaling pathway
Positive regulation of erythrocyte differentiation
Positive regulation of pathway-restricted SMAD protein phosphorylation
Positive regulation of transcription by RNA polymerase II
Positive regulation of trophoblast cell migration
Protein autophosphorylation
Protein phosphorylation
Regulation of transcription, DNA-templated
Signal transduction
Transmembrane receptor protein serine/threonine kinase signaling pathway

Cellular Location

Cell membrane

Involvement in disease

ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis.

Topology

Extracellular: 24-126 aa
Helical: 127-149 aa
Cytoplasmic: 150-505 aa

PTM

Autophosphorylated. Phosphorylated by activin receptor type-2 (ACVR2A or ACVR2B) in response to activin-binding at serine and threonine residues in the GS domain. Phosphorylation of ACVR1B by activin receptor type-2 regulates association with SMAD7.
Ubiquitinated. Level of ubiquitination is regulated by the SMAD7-SMURF1 complex.

Zheng, J., Zhang, T., Han, S., Liu, C., Liu, M., Li, S., & Li, J. (2021). Activin A improves the neurological outcome after ischemic stroke in mice by promoting oligodendroglial ACVR1B-mediated white matter remyelination. Experimental Neurology, 337, 113574.

Boueiz, A., Pham, B., Chase, R., Lamb, A., Lee, S., Naing, Z. Z. C., ... & Castaldi, P. J. (2019). Integrative genomics analysis identifies ACVR1B as a candidate causal gene of emphysema distribution. American journal of respiratory cell and molecular biology, 60(4), 388-398.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ACVR1B Matched Antibody Pair (018) (CAT#: APMAB-018LY)

ACVR1B Matched Antibody Pair (019) (CAT#: APMAB-019LY)

Human Recombinant ACVR1B protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2024)

Human Recombinant ACVR1B protein, GST Tag (CAT#: V2LY-0526-LY2025)

Human Recombinant ACVR1B, Active protein, GST Tag (CAT#: V2LY-0526-LY2026)

Human Recombinant ACVR1B protein, His Tag (CAT#: V2LY-0526-LY2027)

Human Recombinant ACVR1B protein, His Tag-1 (CAT#: V2LY-0526-LY2028)

Mouse Recombinant ACVR1B protein, hFc Tag (CAT#: V2LY-0526-LY7791)

Rhesus Recombinant ACVR1B protein, hFc Tag (CAT#: V2LY-0526-LY9644)

Antibody Pairs

ACVR1B Matched Antibody Pair (018) (CAT#: APMAB-018LY)

ACVR1B Matched Antibody Pair (019) (CAT#: APMAB-019LY)

Related Products

ACVR1B Matched Antibody Pair (019) (CAT#: APMAB-019LY)

Mouse Anti-ACVR1B Recombinant Antibody (V2-179670) (CAT#: CBMAB-A1026-YC)

Human Recombinant ACVR1B protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2024)

Mouse Anti-ACVR1B Recombinant Antibody (V2-179683) (CAT#: CBMAB-A1039-YC)

Human Recombinant ACVR1B, Active protein, GST Tag (CAT#: V2LY-0526-LY2026)

Mouse Recombinant ACVR1B protein, hFc Tag (CAT#: V2LY-0526-LY7791)

Human Recombinant ACVR1B protein, GST Tag (CAT#: V2LY-0526-LY2025)

Human Recombinant ACVR1B protein, His Tag (CAT#: V2LY-0526-LY2027)

Mouse Anti-ACVR1B Recombinant Antibody (V2-179682) (CAT#: CBMAB-A1038-YC)

Mouse Anti-ACVR1B Recombinant Antibody (V2-179671) (CAT#: CBMAB-A1027-YC)

Isotype control

For research use only. Not intended for any clinical use.

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