Rat Recombinant CRP protein, His Tag (V2LY-0526-LY9308)

Name: Rat Recombinant CRP protein, His Tag
SKU: V2LY-0526-LY9308
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant CRP protein, His Tag consist of Amino Acid: 1-230 and predicts a molecular mass of 24.6 kDa.

Molecule Mass

24.6 kDa

Sequence

Amino Acid: 1-230

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

C-Reactive Protein

Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Biological Process

Acute-phase response Source: BHF-UCL
Complement activation, classical pathway Source: GO_Central
Defense response to Gram-positive bacterium Source: BHF-UCL
Inflammatory response Source: ProtInc
Innate immune response Source: GO_Central
Negative regulation by host of viral process Source: GO_Central
Negative regulation of lipid storage Source: BHF-UCL
Negative regulation of macrophage derived foam cell differentiation Source: BHF-UCL
Negative regulation of mononuclear cell proliferation Source: CACAO
Opsonization Source: BHF-UCL
Positive regulation of gene expression Source: AgBase
Positive regulation of superoxide anion generation Source: AgBase
Regulation of interleukin-8 production Source: UniProtKB
Vasoconstriction Source: GO_Central

Cellular Location

Secreted

Luo, X., Zhou, W., Yan, X., Guo, T., Wang, B., Xia, H., ... & Yang, W. (2020). Prognostic value of C-reactive protein in patients with coronavirus 2019. Clinical Infectious Diseases, 71(16), 2174-2179.

Wang, G., Wu, C., Zhang, Q., Wu, F., Yu, B., Lv, J., ... & Zhong, Y. (2020, May). C-reactive protein level may predict the risk of COVID-19 aggravation. In Open forum infectious diseases (Vol. 7, No. 5, p. ofaa153). US: Oxford University Press.

Pathak, A., & Agrawal, A. (2019). Evolution of C-reactive protein. Frontiers in immunology, 10, 943.

Sproston, N. R., & Ashworth, J. J. (2018). Role of C-reactive protein at sites of inflammation and infection. Frontiers in immunology, 9, 754.

Ticinesi, A., Lauretani, F., Nouvenne, A., Porro, E., Fanelli, G., Maggio, M., & Meschi, T. (2017). C-reactive protein (CRP) measurement in geriatric patients hospitalized for acute infection. European Journal of Internal Medicine, 37, 7-12.

Nehring, S. M., & Patel, B. C. (2017). C reactive protein (CRP).

Moutachakkir, M., Baraou, A., Boukhira, A., & Chellak, S. (2017, April). Immunoanalytical characteristics of C-reactive protein and high sensitivity C-reactive protein. In Annales de biologie clinique (Vol. 75, No. 2, pp. 225-229).

Köhler-Forsberg, O., Buttenschøn, H. N., Tansey, K. E., Maier, W., Hauser, J., Dernovsek, M. Z., ... & Mors, O. (2017). Association between C-reactive protein (CRP) with depression symptom severity and specific depressive symptoms in major depression. Brain, behavior, and immunity, 62, 344-350.

Stirling, A. D., Moran, N. R., Kelly, M. E., Ridgway, P. F., & Conlon, K. C. (2017). The predictive value of C-reactive protein (CRP) in acute pancreatitis–is interval change in CRP an additional indicator of severity?. Hpb, 19(10), 874-880.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CRP protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY3363)

Human Recombinant CRP protein, Biotin Conjugated (CAT#: V2LY-0526-LY3364)

Human Recombinant CRP protein (CAT#: V2LY-0526-LY3365)

Mouse Recombinant CRP protein, His Tag (CAT#: V2LY-0526-LY8155)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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