Rat Recombinant ECM1 protein, His Tag (V2LY-0526-LY9341)

Name: Rat Recombinant ECM1 protein, His Tag
SKU: V2LY-0526-LY9341
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant ECM1 protein, His Tag consist of Amino Acid: 1-562 and predicts a molecular mass of 62.9 kDa.

Molecule Mass

62.9 kDa

Sequence

Amino Acid: 1-562

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Extracellular Matrix Protein 1

Research Area

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

Biological Process

Angiogenesis Source: UniProtKB-KW
Biomineral tissue development Source: UniProtKB-KW
Chondrocyte development Source: Ensembl
Endochondral bone growth Source: Ensembl
Inflammatory response Source: Ensembl
Negative regulation of bone mineralization Source: UniProtKB
Negative regulation of cytokine-mediated signaling pathway Source: Ensembl
Negative regulation of peptidase activity Source: UniProtKB
Ossification Source: UniProtKB-KW
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Regulation of bone mineralization Source: GO_Central
Regulation of T cell migration Source: Ensembl
Regulation of transcription by RNA polymerase II Source: Ensembl
Regulation of type 2 immune response Source: Ensembl
Signal transduction Source: InterPro

Cellular Location

Extracellular matrix

Involvement in disease

Lipoid proteinosis (LiP):
Rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.

Jeong, S., Lee, S. G., Kim, H., Lee, G., Park, S., Kim, I. K., ... & Jo, Y. S. (2021). Simultaneous Expression of Long Non-Coding RNA FAL1 and Extracellular Matrix Protein 1 Defines Tumour Behaviour in Young Patients with Papillary Thyroid Cancer. Cancers, 13(13), 3223.

Yin, H., Wang, J., Li, H., Yu, Y., Wang, X., Lu, L., ... & Yang, G. (2021). Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. Nature communications, 12(1), 1-19.

Utsunomiya, N., Utsunomiya, A., Chino, T., Hasegawa, M., & Oyama, N. (2020). Gene silencing of extracellular matrix protein 1 (ECM1) results in phenotypic alterations of dermal fibroblasts reminiscent of clinical features of lichen sclerosus. Journal of Dermatological Science, 100(2), 99-109.

Hardy, S. A., Mabotuwana, N. S., Murtha, L. A., Coulter, B., Sanchez-Bezanilla, S., Al-Omary, M. S., ... & Boyle, A. J. (2019). Novel role of extracellular matrix protein 1 (ECM1) in cardiac aging and myocardial infarction. PloS one, 14(2), e0212230.

Wang, Z., Zhou, Q., Li, A., Huang, W., Cai, Z., & Chen, W. (2019). Extracellular matrix protein 1 (ECM1) is associated with carcinogenesis potential of human bladder cancer. OncoTargets and therapy, 12, 1423.

He, L., Gu, W., Wang, M., Chang, X., Sun, X., Zhang, Y., ... & Sun, B. (2018). Extracellular matrix protein 1 promotes follicular helper T cell differentiation and antibody production. Proceedings of the National Academy of Sciences, 115(34), 8621-8626.

Gao, D., Ma, X., Lian, P., Zhou, S., & Chen, J. (2018). Pathogenetic mechanism of lipoid proteinosis caused by mutation of the extracellular matrix protein 1 gene Retraction in/10.3892/mmr. 2019.9925. Molecular Medicine Reports, 17(6), 8087-8090.

Wu, Q., Chen, D., Luo, Q., Yang, Q., Zhao, C., Zhang, D., ... & Qi, Z. (2018). Extracellular matrix protein 1 recruits moesin to facilitate invadopodia formation and breast cancer metastasis. Cancer Letters, 437, 44-55.

Gan, L., Meng, J., Xu, M., Liu, M., Qi, Y., Tan, C., ... & Wang, Z. (2018). Extracellular matrix protein 1 promotes cell metastasis and glucose metabolism by inducing integrin β4/FAK/SOX2/HIF-1α signaling pathway in gastric cancer. Oncogene, 37(6), 744-755.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ECM1 protein, His Tag (CAT#: V2LY-0526-LY3681)

Mouse Recombinant ECM1 protein, His Tag (CAT#: V2LY-0526-LY8216)

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Isotype control

For research use only. Not intended for any clinical use.

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