Rat Recombinant IGFBP6 protein, His Tag (V2LY-0526-LY9430)

Name: Rat Recombinant IGFBP6 protein, His Tag
SKU: V2LY-0526-LY9430
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant IGFBP6 protein, His Tag consist of Amino Acid: 1-226 and predicts a molecular mass of 22.9 kDa.

Molecule Mass

22.9 kDa

Sequence

Amino Acid: 1-226

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

insulin-like growth factor binding protein 6

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration (PubMed:24003225).

Biological Process

Cell migration Source: UniProtKB
Negative regulation of canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of cell population proliferation Source: ProtInc
Positive regulation of MAPK cascade Source: UniProtKB
Positive regulation of stress-activated MAPK cascade Source: UniProtKB
Regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Secreted

PTM

O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.

Venuto, S., Coda, A. R. D., González-Pérez, R., Laselva, O., Tolomeo, D., Storlazzi, C. T., ... & Conese, M. (2023). IGFBP-6 Network in Chronic Inflammatory Airway Diseases and Lung Tumor Progression. International Journal of Molecular Sciences, 24(5), 4804.

Longhitano, L., Vicario, N., Forte, S., Giallongo, C., Broggi, G., Caltabiano, R., ... & Tibullo, D. (2023). Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma. Cancer Immunology, Immunotherapy, 72(1), 1-20.

Liso, A., Venuto, S., Coda, A. R. D., Giallongo, C., Palumbo, G. A., & Tibullo, D. (2022). IGFBP-6: at the crossroads of immunity, tissue repair and fibrosis. International Journal of Molecular Sciences, 23(8), 4358.

Laselva, O., Criscione, M. L., Allegretta, C., Di Gioia, S., Liso, A., & Conese, M. (2022). Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells. Frontiers in Molecular Biosciences, 9, 905468.

Longhitano, L., Forte, S., Orlando, L., Grasso, S., Barbato, A., Vicario, N., ... & Tibullo, D. (2022). The crosstalk between GPR81/IGFBP6 promotes breast cancer progression by modulating lactate metabolism and oxidative stress. Antioxidants, 11(2), 275.

Longhitano, L., Tibullo, D., Vicario, N., Giallongo, C., La Spina, E., Romano, A., ... & Liso, A. (2021). IGFBP-6/sonic hedgehog/TLR4 signalling axis drives bone marrow fibrotic transformation in primary myelofibrosis. Aging (Albany NY), 13(23), 25055.

Liu, Y., Huan, W., Wu, J., Zou, S., & Qu, L. (2020). IGFBP6 is downregulated in unstable carotid atherosclerotic plaques according to an integrated bioinformatics analysis and experimental verification. Journal of Atherosclerosis and Thrombosis, 27(10), 1068-1085.

Shkurnikov, M., Nikulin, S., Nersisyan, S., Poloznikov, A., Zaidi, S., Baranova, A., ... & Tonevitsky, A. (2019). LAMA4-regulating miR-4274 and its host gene SORCS2 play a role in IGFBP6-dependent effects on phenotype of basal-like breast cancer. Frontiers in molecular biosciences, 6, 122.

Conese, M., D’Oria, S., Castellani, S., Trotta, R., Montemurro, P., & Liso, A. (2018). Insulin-like growth factor-6 (IGFBP-6) stimulates neutrophil oxidative burst, degranulation and chemotaxis. Inflammation Research, 67, 107-109.

Liso, A., Capitanio, N., Gerli, R., & Conese, M. (2018). From fever to immunity: A new role for IGFBP‐6?. Journal of cellular and molecular medicine, 22(10), 4588-4596.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IGFBP6 protein, His Tag (CAT#: V2LY-0526-LY4672)

Mouse Recombinant IGFBP6 protein, His Tag (CAT#: V2LY-0526-LY8442)

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Mouse Recombinant IGFBP6 protein, His Tag (CAT#: V2LY-0526-LY8442)

Human Recombinant IGFBP6 protein, His Tag (CAT#: V2LY-0526-LY4672)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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