Rat Recombinant LAYN protein, His Tag (V2LY-0526-LY9507)

Name: Rat Recombinant LAYN protein, His Tag
SKU: V2LY-0526-LY9507
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant LAYN protein, His Tag consist of Amino Acid: 1-224 and predicts a molecular mass of 24.3 kDa.

Molecule Mass

24.3 kDa

Sequence

Amino Acid: 1-224

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

layilin

Function

Receptor for hyaluronate.

Cellular Location

Membrane
Colocalizes with TLN1 at the membrane ruffles.

Topology

Extracellular: 22-235
Helical: 236-256
Cytoplasmic: 257-382

Liu, C., Wang, Y., Zhang, Y. H., Yuan, Z., Zhang, Z., Zeng, X., ... & Lu, S. (2023). Elevated Layilin-Positive Monocyte Levels in the Peripheral Blood of Patients with Systemic Lupus Erythematosus Reflect Their Autoimmune Status. Immunological Investigations, 52(7), 879-896.

Yang, Y., Chen, Z., Chu, X., Yan, Q., He, J., Guo, Y., ... & Pan, J. (2023). Targeting LAYN inhibits colorectal cancer metastasis and tumor-associated macrophage infiltration induced by hyaluronan oligosaccharides. Matrix Biology, 117, 15-30.

Yang, B., Deng, B., Jiao, X. D., Qin, B. D., Lu, Y., Zhang, W., ... & Zang, Y. S. (2022). Low-dose anti-VEGFR2 therapy promotes anti-tumor immunity in lung adenocarcinoma by down-regulating the expression of layilin on tumor-infiltrating CD8+ T cells. Cellular Oncology, 45(6), 1297-1309.

Li, L., Ma, H., & Song, C. (2022). LAYN acts as a prognostic biomarker in ovarian cancer by engaging T cell exclusion and dysfunction.

Cao, L., Zhu, L., & Cheng, L. (2022). ncRNA-Regulated LAYN Serves as a Prognostic Biomarker and Correlates with Immune Cell Infiltration in Hepatocellular Carcinoma: A Bioinformatics Analysis. BioMed Research International, 2022.

Mehta, P., Gouirand, V., Boda, D. P., Zhang, J., Gearty, S. V., Zirak, B., ... & Rosenblum, M. D. (2021). Layilin anchors regulatory T cells in skin. The Journal of Immunology, 207(7), 1763-1775.

Mahuron, K. M., Moreau, J. M., Glasgow, J. E., Boda, D. P., Pauli, M. L., Gouirand, V., ... & Rosenblum, M. D. (2020). Layilin augments integrin activation to promote antitumor immunity. Journal of Experimental Medicine, 217(9).

Pan, J. H., Zhou, H., Cooper, L., Huang, J. L., Zhu, S. B., Zhao, X. X., ... & Rong, L. (2019). LAYN is a prognostic biomarker and correlated with immune infiltrates in gastric and colon cancers. Frontiers in immunology, 10, 6.

Kim, Y., West, G. A., Ray, G., Kessler, S. P., Petrey, A. C., Fiocchi, C., ... & de la Motte, C. A. (2018). Layilin is critical for mediating hyaluronan 35 kDa-induced intestinal epithelial tight junction protein ZO-1 in vitro and in vivo. Matrix Biology, 66, 93-109.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LAYN protein, hFc Tag (CAT#: V2LY-0526-LY5257)

Human Recombinant LAYN protein, His Tag (CAT#: V2LY-0526-LY5258)

Rat Recombinant LAYN protein (CAT#: V2LY-0526-LY9505)

Rat Recombinant LAYN protein, hFc Tag (CAT#: V2LY-0526-LY9506)

Rhesus Recombinant LAYN protein, hFc Tag (CAT#: V2LY-0526-LY9765)

Rhesus Recombinant LAYN protein, His Tag (CAT#: V2LY-0526-LY9766)

Related Products

Human Recombinant LAYN protein, His Tag (CAT#: V2LY-0526-LY5258)

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Rhesus Recombinant LAYN protein, hFc Tag (CAT#: V2LY-0526-LY9765)

Mouse Anti-LAYN Recombinant Antibody (4F33) (CAT#: CBMAB-L0749-YJ)

Mouse Anti-LAYN Recombinant Antibody (CBYJL-1223) (CAT#: CBMAB-L0740-YJ)

Mouse Anti-LAYN Recombinant Antibody (CBYJL-1222) (CAT#: CBMAB-L0739-YJ)

Human Recombinant LAYN protein, hFc Tag (CAT#: V2LY-0526-LY5257)

Mouse Anti-LAYN Recombinant Antibody (3F7D7E2) (CAT#: CBMAB-L0746-YJ)

Mouse Anti-LAYN Recombinant Antibody (MM0448-4F33) (CAT#: CBMAB-L0753-YJ)

Mouse Anti-LAYN Recombinant Antibody (CBYJL-1232) (CAT#: CBMAB-L0754-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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