Rat Recombinant NCAM1 protein, His Tag (V2LY-0526-LY9525)

Name: Rat Recombinant NCAM1 protein, His Tag
SKU: V2LY-0526-LY9525
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant NCAM1 protein, His Tag consist of Amino Acid: 1-721 and predicts a molecular mass of 79.2 kDa.

Molecule Mass

79.2 kDa

Verified

HPLC

Sequence

Amino Acid: 1-721

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Neural Cell Adhesion Molecule 1

Alternative Names

Neural Cell Adhesion Molecule 1; NCAM; Antigen Recognized By Monoclonal Antibody 5.1H11; Neural Cell Adhesion Molecule, NCAM; CD56 Antigen; N-CAM-1; NCAM-1; MSK39; CD56;

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

(Microbial infection) Acts as a receptor for rabies virus.

(Microbial infection) Acts as a receptor for Zika virus.

Biological Process

Cell adhesion Source: ProtInc
Commissural neuron axon guidance Source: ARUK-UCL
Regulation of semaphorin-plexin signaling pathway Source: ARUK-UCL

Cellular Location

Isoform 1&2&3&4: Cell membrane
Isoform 5&6: Secreted

Topology

Extracellular: 20-718
Helical: 719-739
Cytoplasmic: 740-858

Kim, W., Watanabe, H., Lomoio, S., & Tesco, G. (2021). Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo. Journal of Biological Chemistry, 296.

Caza, T. N., Hassen, S. I., Kuperman, M., Sharma, S. G., Dvanajscak, Z., Arthur, J., ... & Larsen, C. P. (2021). Neural cell adhesion molecule 1 is a novel autoantigen in membranous lupus nephritis. Kidney international, 100(1), 171-181.

Vukojevic, V., Mastrandreas, P., Arnold, A., Peter, F., Kolassa, I. T., Wilker, S., ... & Stetak, A. (2020). Evolutionary conserved role of neural cell adhesion molecule-1 in memory. Translational psychiatry, 10(1), 217.

Jesudas, B. R., Nandeesha, H., Menon, V., & Allimuthu, P. (2020). Relationship of elevated neural cell adhesion molecule 1 with interleukin-10 and disease severity in bipolar disorder. Asian Journal of Psychiatry, 47, 101849.

Kim, Y. H., Kim, Y. H., Shin, Y. K., Jo, Y. R., Park, D. K., Song, M. Y., ... & Park, H. T. (2019). p75 and neural cell adhesion molecule 1 can identify pathologic Schwann cells in peripheral neuropathies. Annals of Clinical and Translational Neurology, 6(7), 1292-1301.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-NCAM1 Recombinant Antibody (CAT#: CBMAB-0526-LY)

Cynomolgus Recombinant CD56 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1605)

Human Recombinant NCAM1 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5699)

Human Recombinant NCAM1 protein, ECD, His Tag (CAT#: V2LY-0526-LY5700)

Human Recombinant NCAM1 protein, hFc Tag (CAT#: V2LY-0526-LY5701)

Human Recombinant NCAM1 protein, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY5702)

Human Recombinant NCAM1 protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY5703)

Human Recombinant NCAM1 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY5704)

Human Recombinant NCAM1 protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY5705)

Mouse Recombinant NCAM1 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8687)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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