Rat Recombinant PEAR1 protein, His Tag (V2LY-0526-LY9552)

Name: Rat Recombinant PEAR1 protein, His Tag
SKU: V2LY-0526-LY9552
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant PEAR1 protein, His Tag consist of Amino Acid: 1-752 and predicts a molecular mass of 78.9 kDa.

Molecule Mass

78.9 kDa

Sequence

Amino Acid: 1-752

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Platelet Endothelial Aggregation Receptor 1

Function

When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.

Biological Process

Phosphatidylinositol 3-kinase signalingIEA:Ensembl
Platelet aggregationIEA:Ensembl
Protein kinase B signalingIEA:Ensembl
Recognition of apoptotic cellIEA:Ensembl

Cellular Location

Cell membrane
Detected on the cell surface in resting platelets.

Topology

Extracellular: 21-755
Helical: 756-776
Cytoplasmic: 777-1037

PTM

Phosphorylated in the intracellular domain on tyrosine residues (By similarity).
Phosphorylated on tyrosine residues by SRC. Tyrosine phosphorylation is detected upon platelet aggregation stimulated by collagen, TRAP and thrombin and platelet-platelet contacts but not after platelet activation. Tyrosine phosphorylation enhanced its association with SHC1 and SHC2.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant PEAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY1313)

Cynomolgus, Rhesus Recombinant PEAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY1737)

Rabbit Recombinant PEAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY9142)

Related Products

Rabbit Recombinant PEAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY9142)

Canine Recombinant PEAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY1313)

Mouse Anti-PEAR1 Recombinant Antibody (12K158) (CAT#: CBMAB-P1400-YC)

Cynomolgus, Rhesus Recombinant PEAR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY1737)

Mouse Anti-PEAR1 Recombinant Antibody (CBYC-P284) (CAT#: CBMAB-P1401-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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