Rat Recombinant UNC5B protein, hFc Tag (V2LY-0526-LY9631)

Name: Rat Recombinant UNC5B protein, hFc Tag
SKU: V2LY-0526-LY9631
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

hFc Tag

Protein Construction

This product is Rat Recombinant UNC5B protein, hFc Tag consist of Amino Acid: 1-373 and predicts a molecular mass of 65.7 kDa.

Molecule Mass

65.7 kDa

Sequence

Amino Acid: 1-373

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Unc-5 Netrin Receptor B

Function

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity).
Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity).
It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906).
Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).

Biological Process

Biological Process angiogenesis Source:UniProtKB-KW
Biological Process anterior/posterior axon guidance Source:GO_Central1 Publication
Biological Process apoptotic process Source:UniProtKB-KW
Biological Process axon guidance Source:GO_Central1 Publication
Biological Process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source:UniProtKB1 Publication
Biological Process negative regulation of neuron apoptotic process Source:UniProtKB1 Publication
Biological Process positive regulation of phosphatidylinositol 3-kinase signaling Source:UniProtKB1 Publication

Cellular Location

Cell membrane
Membrane raft
Associated with lipid rafts.

Topology

Extracellular: 27-377
Helical: 378-398
Cytoplasmic: 399-945

PTM

Phosphorylated on cytoplasmic tyrosine residues.
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant UNC5B protein, ECD, hFc Tag (CAT#: V2LY-0526-LY7548)

Human Recombinant UNC5B protein, ECD, His Tag (CAT#: V2LY-0526-LY7549)

Related Products

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Mouse Anti-UNC5B Recombinant Antibody (1A9) (CAT#: CBMAB-C4663-CN)

Human Recombinant UNC5B protein, ECD, His Tag (CAT#: V2LY-0526-LY7549)

Mouse Anti-UNC5B Recombinant Antibody (187305) (CAT#: CBMAB-C4662-CN)

Human Recombinant UNC5B protein, ECD, hFc Tag (CAT#: V2LY-0526-LY7548)

Rabbit Anti-UNC5B Recombinant Antibody (D9M7Z) (CAT#: CBMAB-C4664-CN)

Mouse Anti-UNC5B Recombinant Antibody (Guido-1) (CAT#: CBMAB-0932CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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