Rhesus Recombinant BTLA protein, His Tag (V2LY-0526-LY9652)

Name: Rhesus Recombinant BTLA protein, His Tag
SKU: V2LY-0526-LY9652
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rhesus

Tag

His Tag

Protein Construction

This product is Rhesus Recombinant BTLA protein, His Tag consist of Amino Acid: 1-155 and predicts a molecular mass of 19.3 kDa.

Molecule Mass

19.3 kDa

Verified

HPLC

Sequence

Amino Acid: 1-155

Species

Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥85%as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

B And T Lymphocyte Associated

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050).
May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044).
In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Immune response-regulating cell surface receptor signaling pathway Source: GO_Central
T cell costimulation Source: Reactome

Cellular Location

Cell membrane

Topology

Extracellular: 31-157 aa
Helical: 158-178 aa
Cytoplasmic: 179-289 aa

PTM

Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.1 Publication
N-glycosylated.

Ning, Z., Liu, K., & Xiong, H. (2021). Roles of BTLA in immunity and immune disorders. Frontiers in Immunology, 12.

Deng, Z., Zheng, Y., Cai, P., & Zheng, Z. (2021). The Role of B and T Lymphocyte Attenuator in Respiratory System Diseases. Frontiers in Immunology, 12, 2141.

Wiedemann, A., Lettau, M., Weißenberg, S. Y., Stefanski, A. L., Schrezenmeier, E. V., Rincon-Arevalo, H., ... & Dörner, T. (2021). BTLA Expression and Function Are Impaired on SLE B Cells. Frontiers in immunology, 12, 1402.

Axelsson, S., Magnuson, A., Lange, A., Alshamari, A., Hörnquist, E. H., & Hultgren, O. (2020). A combination of the activation marker CD86 and the immune checkpoint marker B and T lymphocyte attenuator (BTLA) indicates a putative permissive activation state of B cell subtypes in healthy blood donors independent of age and sex. BMC immunology, 21(1), 1-9.

Zhang, H., Wang, Z., Zhang, J., Zhang, X., Gui, Z., Sun, L., ... & Gu, M. (2020). The synergism of B and T lymphocyte attenuator (BTLA) and cytotoxic T lymphocyte associated antigen-4 (CTLA-4) attenuated acute T-cell mediated rejection and prolonged renal graft survival. Translational Andrology and Urology, 9(5), 1990.

Karabon, L., Partyka, A., Ciszak, L., Pawlak-Adamska, E., Tomkiewicz, A., Bojarska-Junak, A., ... & Kosmaczewska, A. (2020). Abnormal expression of BTLA and CTLA-4 immune checkpoint molecules in chronic lymphocytic leukemia patients. Journal of immunology research, 2020.

Yu, X., Zheng, Y., Mao, R., Su, Z., & Zhang, J. (2019). BTLA/HVEM signaling: milestones in research and role in chronic hepatitis B virus infection. Frontiers in immunology, 10, 617.

Monaghan, S. F., Banerjee, D., Chung, C. S., Lomas-Neira, J., Cygan, K. J., Rhine, C. L., ... & Ayala, A. (2018). Changes in the process of alternative RNA splicing results in soluble B and T lymphocyte attenuator with biological and clinical implications in critical illness. Molecular Medicine, 24(1), 1-7.

Sawaf, M., Fauny, J. D., Felten, R., Sagez, F., Gottenberg, J. E., Dumortier, H., & Monneaux, F. (2018). Defective BTLA functionality is rescued by restoring lipid metabolism in lupus CD4+ T cells. Jci Insight, 3(13).

Lan, X., Li, S., Gao, H., Nanding, A., Quan, L., Yang, C., ... & Xue, Y. (2017). Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. OncoTargets and therapy, 10, 919.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BTLA protein, ECD, Fc Tag (CAT#: V2LY-0526-LY2432)

Human Recombinant BTLA protein, ECD, Fc Tag-1 (CAT#: V2LY-0526-LY2433)

Human Recombinant BTLA protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2434)

Human Recombinant BTLA protein, hFc Tag (CAT#: V2LY-0526-LY2435)

Human Recombinant BTLA protein, hFc Tag-1 (CAT#: V2LY-0526-LY2436)

Human Recombinant BTLA, isoform 1 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2437)

Human Recombinant BTLA protein, His Tag (CAT#: V2LY-0526-LY2438)

Human Recombinant BTLA (isoform 1) protein, Fc Tag (CAT#: V2LY-0526-LY2439)

Human Recombinant BTLA (isoform1) protein, His Tag (CAT#: V2LY-0526-LY2440)

Mouse Recombinant BTLA protein, hFc Tag (CAT#: V2LY-0526-LY7886)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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