Phospho-RAD18 (Ser403)

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

Full Name

RAD18, E3 Ubiquitin Protein Ligase

Function

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Biological Process

Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
DNA repair1 PublicationNAS:UniProtKB
Negative regulation of cell deathManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Postreplication repairManual Assertion Based On ExperimentIBA:GO_Central
Protein autoubiquitinationManual Assertion Based On ExperimentIDA:MGI
Protein monoubiquitinationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Associates with chromatin (PubMed:25931565).
Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077).
Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607).
Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).