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Rat Adipokine Antibody Array (Membrane, 30 Targets) (Y0250) (AbAr-0250-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Rat Adipokine Antibody Array can be used to simultaneously detect the relative levels of 30 different obesity-related molecules in a single sample.
Specifications
Target Array
Adipokine Array
Target
ANGPT-L3, IGFBP-1, LIF, CCL2/MCP-1/JE, IGFBP-2, Lipocalin-2/NGAL, CCL5/RANTES, IGFBP-3, M-CSF, DPPIV/CD26, IGFBP-5, PAI-1/Serpin E1, Endocan, IGFBP-6, Pref-1, FGF-21, IL-1 beta, RAGE, HGF, IL-6, Resistin, ICAM-1, IL-10, TIMP-1, IGF-I, IL-11, TNF-alpha, IGF-II, Leptin, VEGF
Species Reactivity
Rat
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
The main adipokines include leptin, adiponectin, resistin, tumor-necrosis factor, interleukin 6, chemokine (C–C motif) ligand 2, interleukin 10, and transforming growth factor-β. The imbalance between pro- and anti-inflammatory adipokines on adipose tissue results in insulin resistance and the development of metabolic syndrome, type 2 diabetes, and cardiovascular disease.
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For Research Use Only. Not For Clinical Use.