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Mouse Anti-AATF Recombinant Antibody (V2-178869) (CBMAB-A0096-YC)

Provided herein is a Mouse monoclonal antibody against Human Apoptosis Antagonizing Transcription Factor. The antibody can be used for immunoassay techniques, such as ELISA, IF, IHC, WB.
See all AATF antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-178869
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa151-250 from human AATF (AAH00591) with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC3 µg/ml
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apoptosis Antagonizing Transcription Factor
Introduction
AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was
Entrez Gene ID
26574
UniProt ID
Q9NY61
Alternative Names
Apoptosis Antagonizing Transcription Factor; Rb-Binding Protein Che-1; CHE1; DED; Apoptosis-Antagonizing Transcription Factor; Protein AATF; CHE-1; BFR2;
Function
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Biological Process
Cell adhesion
Cellular response to DNA damage stimulus
Embryonic cleavage
Negative regulation of amyloid precursor protein biosynthetic process
Negative regulation of apoptotic process
Negative regulation of apoptotic signaling pathway
Negative regulation of reactive oxygen species metabolic process
Negative regulation of superoxide anion generation
Positive regulation of transcription by RNA polymerase II
Regulation of mitotic cell cycle
Regulation of transcription by RNA polymerase II
Ribosome biogenesis
Cellular Location
Nucleolus
PTM
Hyperphosphorylated during the G1/S phase transition.

Wang, W., Ma, Y. M., Jiang, Z. L., Gao, Z. W., & Chen, W. G. (2021). Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis. Experimental and Therapeutic Medicine, 21(4), 1-1.

Srinivas, A. N., Suresh, D., Mirshahi, F., Santhekadur, P. K., Sanyal, A. J., & Kumar, D. P. (2021). Emerging roles of AATF: checkpoint signaling and beyond. Journal of Cellular Physiology, 236(5), 3383-3395.

Kumar, D. P., Santhekadur, P. K., Seneshaw, M., Mirshahi, F., Uram‐Tuculescu, C., & Sanyal, A. J. (2019). A regulatory role of apoptosis antagonizing transcription factor in the pathogenesis of nonalcoholic fatty liver disease and hepatocellular carcinoma. Hepatology, 69(4), 1520-1534.

Jain, M., Kaiser, R. W., Bohl, K., Hoehne, M., Göbel, H., Bartram, M. P., ... & Slaats, G. G. (2019). Inactivation of apoptosis antagonizing transcription factor in tubular epithelial cells induces accumulation of DNA damage and nephronophthisis. Kidney international, 95(4), 846-858.

Fratini, E., Cervelli, M., Mariottini, P., Kanamori, Y., Amendola, R., & Agostinelli, E. (2019). Link between spermine oxidase and apoptosis antagonizing transcription factor: A new pathway in neuroblastoma. International journal of oncology, 55(5), 1149-1156.

Benakanakere, M. R., Zhao, J., Finoti, L., Schattner, R., Odabas-Yigit, M., & Kinane, D. F. (2019). MicroRNA-663 antagonizes apoptosis antagonizing transcription factor to induce apoptosis in epithelial cells. Apoptosis, 24(1), 108-118.

Jing, P., Zou, J., Weng, K., & Peng, P. (2018). The PI 3K/AKT axis modulates AATF activity in Wilms’ tumor cells. FEBS Open bio, 8(10), 1615-1623.

Jain, M. (2018). Targeted deletion of the DNA damage response regulator Apoptosis Antagonizing Transcription Factor (AATF) in mice decelerates lung tumorigenesis and causes degenerative renal disease (Doctoral dissertation, Universität zu Köln).

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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