Mouse Anti-AATF Recombinant Antibody (V2-178870) (CBMAB-A0097-YC)

Name: Mouse Anti-AATF Recombinant Antibody (V2-178870)
SKU: CBMAB-A0097-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-178870

Application

Immunogen

Partial sequence of recombinant full-length protein to human AATF

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide

Buffer

PBS, pH7.3, 1% BSA

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apoptosis Antagonizing Transcription Factor

Introduction

AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was

Entrez Gene ID

26574

UniProt ID

Q9NY61

Alternative Names

Apoptosis Antagonizing Transcription Factor; Rb-Binding Protein Che-1; CHE1; DED; Apoptosis-Antagonizing Transcription Factor; Protein AATF; CHE-1; BFR2;

Function

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.

Biological Process

Cell adhesion
Cellular response to DNA damage stimulus
Embryonic cleavage
Negative regulation of amyloid precursor protein biosynthetic process
Negative regulation of apoptotic process
Negative regulation of apoptotic signaling pathway
Negative regulation of reactive oxygen species metabolic process
Negative regulation of superoxide anion generation
Positive regulation of transcription by RNA polymerase II
Regulation of mitotic cell cycle
Regulation of transcription by RNA polymerase II
Ribosome biogenesis

Cellular Location

Nucleolus

PTM

Hyperphosphorylated during the G1/S phase transition.

Wang, W., Ma, Y. M., Jiang, Z. L., Gao, Z. W., & Chen, W. G. (2021). Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis. Experimental and Therapeutic Medicine, 21(4), 1-1.

Srinivas, A. N., Suresh, D., Mirshahi, F., Santhekadur, P. K., Sanyal, A. J., & Kumar, D. P. (2021). Emerging roles of AATF: checkpoint signaling and beyond. Journal of Cellular Physiology, 236(5), 3383-3395.

Kumar, D. P., Santhekadur, P. K., Seneshaw, M., Mirshahi, F., Uram‐Tuculescu, C., & Sanyal, A. J. (2019). A regulatory role of apoptosis antagonizing transcription factor in the pathogenesis of nonalcoholic fatty liver disease and hepatocellular carcinoma. Hepatology, 69(4), 1520-1534.

Jain, M., Kaiser, R. W., Bohl, K., Hoehne, M., Göbel, H., Bartram, M. P., ... & Slaats, G. G. (2019). Inactivation of apoptosis antagonizing transcription factor in tubular epithelial cells induces accumulation of DNA damage and nephronophthisis. Kidney international, 95(4), 846-858.

Fratini, E., Cervelli, M., Mariottini, P., Kanamori, Y., Amendola, R., & Agostinelli, E. (2019). Link between spermine oxidase and apoptosis antagonizing transcription factor: A new pathway in neuroblastoma. International journal of oncology, 55(5), 1149-1156.

Benakanakere, M. R., Zhao, J., Finoti, L., Schattner, R., Odabas-Yigit, M., & Kinane, D. F. (2019). MicroRNA-663 antagonizes apoptosis antagonizing transcription factor to induce apoptosis in epithelial cells. Apoptosis, 24(1), 108-118.

Jing, P., Zou, J., Weng, K., & Peng, P. (2018). The PI 3K/AKT axis modulates AATF activity in Wilms’ tumor cells. FEBS Open bio, 8(10), 1615-1623.

Jain, M. (2018). Targeted deletion of the DNA damage response regulator Apoptosis Antagonizing Transcription Factor (AATF) in mice decelerates lung tumorigenesis and causes degenerative renal disease (Doctoral dissertation, Universität zu Köln).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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