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Rabbit Anti-ABAT Recombinant Antibody (V2-12485) (CBMAB-0975-CN)

This product is a rabbit antibody that recognizes ABAT of human. The antibody EPR4433 can be used for immunoassay techniques such as: FC, IHC-P, IP, WB.
See all ABAT antibodies
Published Data
Rabbit Anti-ABAT Recombinant Antibody (EPR4433) in WB
ABAT protein level was detected by western blot analysis. Upper panel: a representative western blot for ABAT and GAPDH from 4 out of 7 different hearts in each group. Bottom panel: quantification of ABAT/GAPDH ratio. Data are mean ± SD, n = 7 per group. * p < 0.05 vs. ND. ...View More
Zhang, M., Zhong, H., Cao, T., Huang, Y., Ji, X., Fan, G. C., & Peng, T. (2022). Gamma-aminobutyrate transaminase protects against lipid overload-triggered cardiac injury in mice. International Journal of Molecular Sciences, 23(4), 2182.
Rabbit Anti-ABAT Recombinant Antibody (EPR4433) in WB
Blots of PDK4, HMGcs2, BDH1, ABAT, Ponceau loading control, and graph of quantifications adjusted for loading. Mean + SEM, ∗ = p < 0.05 by ∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.0001. ...View More
Joseph, L. C., Shi, J., Nguyen, Q. N., Pensiero, V., Goulbourne, C., Bauer, R. C., ... & Morrow, J. P. (2022). Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. Iscience, 25(5), 104184.
Rabbit Anti-ABAT Recombinant Antibody (EPR4433) in IF
Distribution of GABA-T in hypothalamus. Arcuate nucleus: ARC; Paraventricular nucleus: PVN; Periventricular nucleus: PeN; Third ventricle: 3V; a1-4: Infancy(10d); b1-4: Prepuberty (21d); c1-4: Prepuberty (28d); d1-4: Puberty (33.67 ± 0.49 d); e1-4: Adult (64.16 ± 0.79 d); f1-4: Negative control; The scale bar of 1, 2, 3, 4 are 250 μm, 100 μm, 100 μm, 50 μm. ...View More
Si, W., Li, H., Kang, T., Ye, J., Yao, Z., Liu, Y., ... & Fang, F. (2020). Effect of gaba-t on reproductive function in female rats. Animals, 10(4), 567.
Rabbit Anti-ABAT Recombinant Antibody (EPR4433) in WB
miR-210 inhibitor prohibited epilepsy-induced changes of GABA-related protein expressions.

Abbreviations: E, epilepsy; GABAT, GABA transaminase; GAD, glutamate decarboxylase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GAT-1, GABA transporter 1; miRI + E, miR-210 inhibitor and epilepsy; V + E, vehicle and epilepsy. ...View More
Chen, L., Zheng, H., & Zhang, S. (2016). Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatric disease and treatment, 1731-1737.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-12485
Antibody Isotype
IgG
Application
IHC-F, IHC-P, IP, WB

Basic Information

Immunogen
A synthetic peptide corresponding to residues in Human ABAT.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:10,000-1:50,000
IP1:10-1:100
IHC-P1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
49% PBS, 0.05% BSA, 50% Glycerol
Preservative
0.01% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
4-Aminobutyrate Aminotransferase
Introduction
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. The protein catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. It can also convert delta-aminovalerate and beta-alanine.
Entrez Gene ID
Human18
Mouse268860
Rat81632
UniProt ID
HumanP80404
MouseP61922
RatP50554
Alternative Names
GABAT; NPD009; GABA-AT
Function
Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Biological Process
Behavioral response to cocaine
Gamma-aminobutyric acid catabolic process
Gamma-aminobutyric acid metabolic process
Neurotransmitter catabolic process
Cellular Location
Mitochondrion matrix
Involvement in disease
An enzymatic deficiency resulting in psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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