Mouse Anti-ACO1 Recombinant Antibody (V2-610925) (CBMAB-A0068-LY)

The product is antibody recognizes ACO1. The antibody 2F6 immunoassay techniques such as: WB, ELISA.
See all ACO1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-610925

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

ACO1 (AAH18103, 780 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Aconitase 1

Introduction

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq]

Entrez Gene ID

Alternative Names

ACONS; IREB1; IREBP; IREBP1; IRP1

Function

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

Biological Process

Cellular iron ion homeostasis
Citrate metabolic process
Intestinal absorption
Post-embryonic development
Regulation of translation
Response to iron(II) ion
Tricarboxylic acid cycle

Cellular Location

Cytoplasm

References

Eprintsev, A. T., Fedorin, D. N., Cherkasskikh, M. V., & Igamberdiev, A. U. (2021). Effect of Salt Stress on the Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Aconitase and Fumarase in Maize. International Journal of Molecular Sciences, 22(11), 6012.

Eprintsev, A. T., Fedorin, D. N., Cherkasskikh, M. V., & Igamberdiev, A. U. (2020). Regulation of expression of the mitochondrial and cytosolic forms of aconitase in maize leaves via phytochrome. Plant Physiology and Biochemistry, 146, 157-162.

De Bellis, L., Luvisi, A., & Alpi, A. (2020). Aconitase: to be or not to be inside plant glyoxysomes, that is the question. Biology, 9(7), 162.

Chen, Y., Cai, G. H., Xia, B., Wang, X., Zhang, C. C., Xie, B. C., ... & Wu, J. W. (2020). Mitochondrial aconitase controls adipogenesis through mediation of cellular ATP production. The FASEB Journal, 34(5), 6688-6702.

Gonzalez-Sanchez, L., Cobos-Fernandez, M. A., Lopez-Nieva, P., Villa-Morales, M., Stamatakis, K., Cuezva, J. M., ... & Fernandez-Piqueras, J. (2020). Exploiting the passenger ACO1-deficiency arising from 9p21 deletions to kill T-cell lymphoblastic neoplasia cells. Carcinogenesis, 41(8), 1113-1122.

Oskarsson, G. R., Oddsson, A., Magnusson, M. K., Kristjansson, R. P., Halldorsson, G. H., Ferkingstad, E., ... & Stefansson, K. (2020). Predicted loss and gain of function mutations in ACO1 are associated with erythropoiesis. Communications biology, 3(1), 1-10.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

ACO1 Matched Antibody Pair (005)

Related Products

Mouse Anti-ACO1 Recombinant Antibody (V2-179324)

ACO1 Matched Antibody Pair (005)

Rabbit Anti-ACO1 Recombinant Antibody (V2-621657)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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