Rabbit Anti-ACO1 Recombinant Antibody (V2-621657) (CBMAB-CP1222-LY)

Name: Rabbit Anti-ACO1 Recombinant Antibody (V2-621657)
SKU: CBMAB-CP1222-LY
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-ACO1 Recombinant Antibody (D6S4J) (CBMAB-CP1222-LY) in IF

HeLa cells stably expressed sh-IRP1 were transiently transfected with mt-Keima. Following 24 h of expression, cells were treated with DFO for another 24 h. ...View More

Wu, H., Wei, H., Zhang, D., Sehgal, S. A., Zhang, D., Wang, X., ... & Chen, Q. (2020). Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy. Redox Biology, 36, 101661.

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

V2-621657

Antibody Isotype

IgG

Application

Basic Information

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu734 of human IRP1 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Aconitase 1

Introduction

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

Entrez Gene ID

UniProt ID

P21399

Alternative Names

Aconitase 1; Iron-Responsive Element-Binding Protein 1; Aconitate Hydratase; Cytoplasmic; Ferritin Repressor Protein; Iron Regulatory Protein 1; Cytosplasmic Aconitase; Aconitase 1; Soluble; Citrate Hydro-Lyase; Soluble Aconitase; EC 4.2.1.3; IRE-BP 1;

Function

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

Biological Process

Cellular iron ion homeostasis
Citrate metabolic process
Intestinal absorption
Post-embryonic development
Regulation of translation
Response to iron(II) ion
Tricarboxylic acid cycle

Cellular Location

Cytoplasm

More Infomation

References

Eprintsev, A. T., Fedorin, D. N., Cherkasskikh, M. V., & Igamberdiev, A. U. (2021). Effect of Salt Stress on the Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Aconitase and Fumarase in Maize. International Journal of Molecular Sciences, 22(11), 6012.

Eprintsev, A. T., Fedorin, D. N., Cherkasskikh, M. V., & Igamberdiev, A. U. (2020). Regulation of expression of the mitochondrial and cytosolic forms of aconitase in maize leaves via phytochrome. Plant Physiology and Biochemistry, 146, 157-162.

De Bellis, L., Luvisi, A., & Alpi, A. (2020). Aconitase: to be or not to be inside plant glyoxysomes, that is the question. Biology, 9(7), 162.

Chen, Y., Cai, G. H., Xia, B., Wang, X., Zhang, C. C., Xie, B. C., ... & Wu, J. W. (2020). Mitochondrial aconitase controls adipogenesis through mediation of cellular ATP production. The FASEB Journal, 34(5), 6688-6702.

Gonzalez-Sanchez, L., Cobos-Fernandez, M. A., Lopez-Nieva, P., Villa-Morales, M., Stamatakis, K., Cuezva, J. M., ... & Fernandez-Piqueras, J. (2020). Exploiting the passenger ACO1-deficiency arising from 9p21 deletions to kill T-cell lymphoblastic neoplasia cells. Carcinogenesis, 41(8), 1113-1122.

Oskarsson, G. R., Oddsson, A., Magnusson, M. K., Kristjansson, R. P., Halldorsson, G. H., Ferkingstad, E., ... & Stefansson, K. (2020). Predicted loss and gain of function mutations in ACO1 are associated with erythropoiesis. Communications biology, 3(1), 1-10.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

ACO1 Matched Antibody Pair (005)

Related Products

ACO1 Matched Antibody Pair (005)

Mouse Anti-ACO1 Recombinant Antibody (V2-610925)

Mouse Anti-ACO1 Recombinant Antibody (V2-179324)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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