Rabbit Anti-ACTR3 Recombinant Antibody (
7E5) (V2LY-0725-LY1049)

Name: Rabbit Anti-ACTR3 Recombinant Antibody (7E5)
SKU: V2LY-0725-LY1049
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ACTR3 Recombinant Antibody (7E5) (V2LY-0725-LY1049) in WB

Western Blot
Positive WB detected in: PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate, Mouse Spleen whole cell lysate, Rat Spleen whole cell lysate
All lanes: Arp3 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7E5

Application

ELISA, WB

Immunogen

A synthesized peptide derived from human Arp3.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ARP3 Actin Related Protein 3 Homolog

Entrez Gene ID

10096

UniProt ID

P61158

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.

Biological Process

Actin polymerization-dependent cell motility
Arp2/3 complex-mediated actin nucleation
Asymmetric cell division
Cellular response to interferon-gamma
Cilium assembly
Ephrin receptor signaling pathway
Establishment or maintenance of cell polarity
Fc-gamma receptor signaling pathway involved in phagocytosis
Meiotic chromosome movement towards spindle pole
Meiotic cytokinesis
Membrane organization
Positive regulation of lamellipodium assembly
Positive regulation of transcription by RNA polymerase II
Spindle localization

Cellular Location

Cytoskeleton; Nucleus; Cell projection. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).

Obeidy, P., Ju, L. A., Oehlers, S. H., Zulkhernain, N. S., Lee, Q., Galeano Niño, J. L., ... & Weninger, W. (2020). Partial loss of actin nucleator actin‐related protein 2/3 activity triggers blebbing in primary T lymphocytes. Immunology and cell biology, 98(2), 93-113.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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