Mouse Anti-ACTR3 Recombinant Antibody (V2-179661) (CBMAB-A1014-YC)

Name: Mouse Anti-ACTR3 Recombinant Antibody (V2-179661)
SKU: CBMAB-A1014-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-179661

Application

WB, ELISA, ICC, IF

Immunogen

Recombinant human ARP3.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Monkey

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.25-0.5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Preservative

15 mM sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ARP3 Actin Related Protein 3 Homolog

Introduction

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamel

Entrez Gene ID

Human	10096
Mouse	74117
Rat	81732
Dog	403702
Monkey	709836

UniProt ID

Human	P61158
Mouse	Q99JY9
Rat	Q4V7C7
Dog	J9PAK3
Monkey	H9FNE9

Alternative Names

ARP3 Actin Related Protein 3 Homolog; Actin-Like Protein 3; ARP3; ARP3 (Actin-Related Protein 3, Yeast) Homolog; ARP3 Actin-Related Protein 3 Homolog (Yeast); Actin-Related Protein 3;

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.

Biological Process

Actin polymerization-dependent cell motility
Arp2/3 complex-mediated actin nucleation
Asymmetric cell division
Cellular response to interferon-gamma
Cilium assembly
Ephrin receptor signaling pathway
Establishment or maintenance of cell polarity
Fc-gamma receptor signaling pathway involved in phagocytosis
Meiotic chromosome movement towards spindle pole
Meiotic cytokinesis
Membrane organization
Positive regulation of lamellipodium assembly
Positive regulation of transcription by RNA polymerase II
Spindle localization

Cellular Location

Cytoskeleton; Nucleus; Cell projection. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).

Obeidy, P., Ju, L. A., Oehlers, S. H., Zulkhernain, N. S., Lee, Q., Galeano Niño, J. L., ... & Weninger, W. (2020). Partial loss of actin nucleator actin‐related protein 2/3 activity triggers blebbing in primary T lymphocytes. Immunology and cell biology, 98(2), 93-113.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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