How is ADD1 specificity guaranteed against the homologous subunits ADD2 (β-adducin) and ADD3 (γ-adducin)?

ADD1 (α-adducin) shares significant sequence homology with ADD2 and ADD3, yet its recombinant antibody is designed to target an epitope located within a divergent region, likely a subunit-specific loop domain or the C-terminal tail. The recombinant approach ensures that the cloned antibody sequence is specific, eliminating the non-selective binding that often occurs with polyclonal antibodies or unverified hybridomas against this protein family.

Is this antibody validated to detect total ADD1 protein regardless of its phosphorylation status?

Yes, this monoclonal antibody is designed to recognize total ADD1 protein, meaning its epitope is not a known phosphorylation site (e.g., Ser726, Thr445). This is crucial for researchers who study ADD1 regulation by PKC and PKA, as it provides a stable baseline for quantifying protein expression levels while parallel experiments monitor the phosphorylation-induced functional changes using site-specific phospho-antibodies.

Mouse Anti-ADD1 Recombinant Antibody (V2-179871) (CBMAB-A1250-YC)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-179871

Application

ELISA, IHC, WB

Immunogen

Full length recombinant corresponding to aa1-662 from human ADD1 (AAH42998) with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-662

More Infomation

Target

Full Name

Adducin 1

Introduction

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-add

Entrez Gene ID

118

UniProt ID

P35611

Alternative Names

Adducin 1; Adducin 1 (Alpha); Alpha-Adducin; ADDA; Erythrocyte Adducin Alpha Subunit; Erythrocyte Adducin Subunit Alpha;

Function

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Biological Process

Actin cytoskeleton organization
Actin filament bundle assembly
Barbed-end actin filament capping
Cell morphogenesis
Cellular response to calcium ion
Cell volume homeostasis
Erythrocyte differentiation
Hemoglobin metabolic process
Homeostasis of number of cells within a tissue
In utero embryonic development
IRE1-mediated unfolded protein response
Multicellular organism growth
Positive regulation of adherens junction organization
Positive regulation of establishment of endothelial barrier
Positive regulation of protein binding
Transmembrane transport

Cellular Location

Cell membrane; Cytoskeleton

Yermolenko, S., Chumachenko, Y., Orlovskyi, V., Moiseyenko, I., & Orlovskyi, O. (2021). The Association between Gly460Trp-Polymorphism of Alpha-Adducin 1 Gene (ADD1) and Arterial Hypertension Development in Ukrainian Population. International Journal of Hypertension, 2021.

Su, C. Y., Yan, R. L., Hsu, W. H., Chu, C. T., Chang, H. C., Lai, C. C., ... & Chen, H. C. (2019). Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration. Scientific reports, 9(1), 1-11.

Gupta, S., Jhawat, V., Agarwal, B. K., Roy, P., & Saini, V. (2019). Alpha adducin (ADD1) gene polymorphism and new onset of diabetes under the influence of selective antihypertensive therapy in essential hypertension. Current hypertension reviews, 15(2), 123-134.

Hsu, W. H., Wang, W. J., Lin, W. Y., Huang, Y. M., Lai, C. C., Liao, J. C., & Chen, H. C. (2018). Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2. EMBO reports, 19(8), e45607.

Park, M. Y., & Lee, M. S. (2018). Effects of Sodium Intake on the Association between the Salt-Sensitive Gene, Alpha-Adducin 1 (ADD1), and Inflammatory Cytokines in the Prevalence of Children Obesity. Journal of Lipid and Atherosclerosis, 7(2), 98-109.

Bayoumy, N. M., El-Shabrawi, M. M., Leheta, O. F., & Omar, H. H. (2017). α-Adducin gene promoter DNA methylation and the risk of essential hypertension. Clinical and Experimental Hypertension, 39(8), 764-768.

Luo, C., & Shen, J. (2017). Adducin in tumorigenesis and metastasis. Oncotarget, 8(29), 48453.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ADD1 Matched Antibody Pair (025) (CAT#: APMAB-025LY)

Related Products

Rabbit Anti-ADD1 Recombinant Antibody (V2-179872) (CAT#: CBMAB-A1252-YC)

Mouse Anti-ADD1 Recombinant Antibody (CBT2090) (CAT#: V2LY-0625-LY243)

ADD1 Matched Antibody Pair (025) (CAT#: APMAB-025LY)

Mouse Anti-ADD1 Recombinant Antibody (V2-12523) (CAT#: CBMAB-1013-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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