Mouse Anti-ADIPOQ Recombinant Antibody (V2-6089) (CBMAB-0034CQ)

Name: Mouse Anti-ADIPOQ Recombinant Antibody (V2-6089)
SKU: CBMAB-0034CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ADIPOQ Recombinant Antibody (19F1) (CBMAB-0034CQ) in WB

WB analysis of serum adiponectin levels in Adipoq-Cre or control (WT) mice injected with AAV2/8-FLEX-Becn1F121A and then fed with HFD for 12 weeks. n = 4. t test. ...View More

Kuramoto, K., Kim, Y. J., Hong, J. H., & He, C. (2021). The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding. Cell reports, 35(8), 109184.

Mouse Anti-ADIPOQ Recombinant Antibody (19F1) (CBMAB-0034CQ) in WB

Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI, WPH, BPI or BPH. Immunoblotting and densitometric analysis of (A,B) PPARγ, (A,C) C/EBPα, and (A,D) adiponectin (n = 6). mRNA levels of PPARγ (E) and PPARγ target genes, (F) adiponectin and (G) stearoyl-CoA desaturase (Scd1) (n = 8) were determined. TG levels (H) were measured using a targeted lipidomics approach in adipocytes incubated with BSA protein isolate or whey peptides (n = 8). (B-H): * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. protein isolate controls. BPI, BSA protein isolate; BPH, BSA peptide hydrolysate; WPI, whey protein isolate; WPH, whey peptide hydrolysate; PPARγ, peroxisome proliferator-activated receptor γ; C/EBPα, CCAAT/enhancer-binding protein α; PS, protein stain; Scd1, stearoyl-Coenzyme A desaturase 1; A.U., arbitrary units. ...View More

D'Souza, K., Mercer, A., Mawhinney, H., Pulinilkunnil, T., Udenigwe, C. C., & Kienesberger, P. C. (2020). Whey peptides stimulate differentiation and lipid metabolism in adipocytes and ameliorate lipotoxicity-induced insulin resistance in muscle cells. Nutrients, 12(2), 425.

Mouse Anti-ADIPOQ Recombinant Antibody (19F1) (CBMAB-0034CQ) in WB

Western blot analysis for total lung expression of adiponectin (26 kDa), AdipoR1 (43 kDa), and AdipoR2 (44 kDa) normalized to β-actin in the newborn rat during alveolarization (postnatal days (PD) 4, 10 and 21). APN is adiponectin, AdR1 and AdR2 are adiponectin receptor 1 and 2, respectively. ...View More

Kang, N. Y., Ivanovska, J., Tamir-Hostovsky, L., Belik, J., & Gauda, E. B. (2018). Chronic intermittent hypoxia in premature infants: The link between low fat stores, adiponectin receptor signaling and lung injury. In Arterial Chemoreceptors: New Directions and Translational Perspectives (pp. 151-157). Springer International Publishing.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-6089

Application

ELISA, ICC, IF, IHC-P, WB

Immunogen

Recombinant Human full length protein

Host Species

Mouse

Specificity

Human, Mouse, Rat, Rabbit, Baboon

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:2,000
FC	1:10-1:1,000
IF(ICC)	2-4 μg/ml
IHC	1:20-1:100
IHC-P	1:10-1:500
WB	1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 1 mg/ml BSA

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adiponectin, C1Q And Collagen Domain Containing

Introduction

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Adiponectin is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adipocytes also produce and secrete proteins such as leptin, adipsin, various other complement components, and tumor necrosis factor, suggesting a possible link to the immune system. Adiponectin, an adipose tissue-specific plasma protein, has antiinflammatory effects on the cellular components of the vascular wall Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Entrez Gene ID

Human	9370
Mouse	11450
Rat	246253
Rabbit	100009027
Baboon	101014121

UniProt ID

Human	Q15848
Mouse	Q60994
Rat	Q8K3R4
Rabbit	G1TV17
Baboon	A0A2I3LW74

Alternative Names

Adiponectin, C1Q And Collagen Domain Containing; Adipose Most Abundant Gene Transcript 1 Protein; Adipocyte Complement-Related 30 KDa Protein; 30 KDa Adipocyte Complement-Related Protein; ACRP30; APM-1; GBP28; ACDC; APM1; Adipocyte, C1q And Collagen Domain-Containing Protein; Adipocyte, C1Q And Collagen Domain Containing; Adipose Most Abundant Gene Transcript 1; Adipose Specific Collagen-Like Factor; Gelatin-Binding Protein 28; Gelatin-Binding Protein; Adiponectin Precursor; ADIPQTL1; ADPN

Function

Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).

Biological Process

Adiponectin-activated signaling pathway
Fatty acid oxidation
Glucose homeostasis
Hormone-mediated signaling pathway
Leptin-mediated signaling pathway
Negative regulation of cell growth
Negative regulation of epithelial cell migration
Negative regulation of epithelial to mesenchymal transition
Negative regulation of NIK/NF-kappaB signaling
Negative regulation of receptor signaling pathway via JAK-STAT
Positive regulation of cold-induced thermogenesis
Positive regulation of insulin receptor signaling pathway
Positive regulation of receptor signaling pathway via JAK-STAT
Regulation of fatty acid biosynthetic process
Regulation of glucose metabolic process
Regulation of lipid metabolic process

Cellular Location

Cell membrane. Localized to the cell membrane and intracellular organelles.

Topology

Cytoplasmic: 1-136 aa
Helical: 137-157 aa
Extracellular: 158-170 aa
Helical: 171-191 aa
Cytoplasmic: 192-203 aa
Helical: 204-224 aa
Extracellular: 225-234 aa
Helical: 235-255 aa
Cytoplasmic: 256-264 aa
Helical: 265-285 aa
Extracellular: 286-298 aa
Helical: 299-319 aa
Cytoplasmic: 320-337 aa
Helical: 338-358 aa
Extracellular: 359-375 aa

Mamoor, S. (2021). Differential expression of adiponectin, C1Q and collagen domain containing in cancers of the breast.

Wang, Q., Ren, D., Bi, Y., Yuan, R., Li, D., Wang, J., ... & Liu, B. (2020). Association and functional study between ADIPOQ and metabolic syndrome in elderly Chinese Han population. Aging (Albany NY), 12(24), 25819.

Coutinho, C. A. A. C., Marson, F. A. L., Ribeiro, J. D., & Bertuzzo, C. S. (2020). A negative screening of rare genetic variants in the ADIPOQ and STATH genes in cystic fibrosis. Pulmonology, 26(3), 138-144.

Chung, S. J., Nagaraju, G. P., Nagalingam, A., Muniraj, N., Kuppusamy, P., Walker, A., ... & Sharma, D. (2017). ADIPOQ/adiponectin induces cytotoxic autophagy in breast cancer cells through STK11/LKB1-mediated activation of the AMPK-ULK1 axis. Autophagy, 13(8), 1386-1403.

Ye, L., Wang, G., Tang, Y., & Bai, J. (2017). A population-specific correlation between ADIPOQ rs2241766 and rs 1501299 and colorectal cancer risk: a meta-analysis for debate. International journal of clinical oncology, 22(2), 307-315.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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