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Mouse Anti-ADK Recombinant Antibody (V2-179987) (CBMAB-A1373-YC)

Provided herein is a Mouse monoclonal antibody against Human Adenosine Kinase. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ADK antibodies
Published Data
Mouse Anti-ADK Recombinant Antibody (H-1) (CBMAB-A1373-YC) in WB
Effect of bortezomib (Bort); 0.75 mg/kg) on ABT-702-induced reduction in cardiac ADK protein levels measured by western blot and normalized to GAPDH (n = 5). * indicates p < .05 comparing vehicle and ABT-702. † indicates p < .05 comparing ABT-702 vs ABT-702 plus bortezomib. ...View More
Wölkart, G., Stessel, H., Fassett, E., Teschl, E., Friedl, K., Trummer, M., ... & Fassett, J. (2022). Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection. European Journal of Pharmacology, 927, 175050.
Mouse Anti-ADK Recombinant Antibody (H-1) (CBMAB-A1373-YC) in Immunoblot
HEK293T cells were treated with ADK specific siRNA. Twelve hours later, cells were transfected with CoV-Gluc, nsp12, nsp7, nsp8 plasmids. Cells were split for immunoblotting detection. After 24 h incubation, Gluc activity was determined. Data represent the mean ± standard deviation (error bars) of the results of three independent experiments. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001. ...View More
Zhao, J., Liu, Q., Yi, D., Li, Q., Guo, S., Ma, L., ... & Cen, S. (2022). 5-Iodotubercidin inhibits SARS-CoV-2 RNA synthesis. Antiviral Research, 198, 105254.
Mouse Anti-ADK Recombinant Antibody (H-1) (CBMAB-A1373-YC) in IHC
IHC staining for Trop2, TALDO1, GPI, LDHA, SHMT2, and ADK in HCC1806 shCTL and shTrop2 xenografts. Scale bar represents 20 µm. ...View More
Aslan, M., Hsu, E. C., Garcia-Marques, F. J., Bermudez, A., Liu, S., Shen, M., ... & Stoyanova, T. (2021). Oncogene-mediated metabolic gene signature predicts breast cancer outcome. NPJ breast cancer, 7(1), 141.
Mouse Anti-ADK Recombinant Antibody (H-1) (CBMAB-A1373-YC) in Immunoblot
Tsc2+/+ Trp53−/− and Tsc2−/− Trp53−/− MEFs 48 hr post-transfection with control or ADK siRNAs were analyzed by immunoblotting. ...View More
Valvezan, A. J., Turner, M., Belaid, A., Lam, H. C., Miller, S. K., McNamara, M. C., ... & Manning, B. D. (2017). mTORC1 couples nucleotide synthesis to nucleotide demand resulting in a targetable metabolic vulnerability. Cancer cell, 32(5), 624-638.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-179987
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Specific for an epitope mapping between amino acids 232-257 within an internal region of ADK of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adenosine Kinase
Introduction
ADK catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervou
Entrez Gene ID
Human132
Mouse11534
Rat25368
UniProt ID
HumanP55263
MouseP55264
RatQ64640
Alternative Names
Adenosine Kinase; Adenosine 5-Phosphotransferase; EC 2.7.1.20; AK; Testicular Tissue Protein Li 14; EC 2.7.1;
Function
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Biological Process
AMP salvage
dATP biosynthetic process
Purine-containing compound salvage
Purine ribonucleoside salvage
Ribonucleoside monophosphate biosynthetic process
Cellular Location
Isoform 1: Nucleus
Isoform 2: Cytoplasm
Involvement in disease
Hypermethioninemia due to adenosine kinase deficiency (HMAKD): A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal.

Wang, Y., Xu, Y., Yan, S., Cao, K., Zeng, X., Zhou, Y., ... & Huo, Y. (2021). Adenosine kinase is critical for neointima formation after vascular injury by inducing aberrant DNA hypermethylation. Cardiovascular research, 117(2), 561-575.

Murugan, M., Fedele, D., Millner, D., Alharfoush, E., Vegunta, G., & Boison, D. (2021). Adenosine kinase: An epigenetic modulator in development and disease. Neurochemistry International, 147, 105054.

Becker, P. H., Demir, Z., Glassberg, Y. M., Sevin, C., Habes, D., Imbard, A., ... & Gaignard, P. (2021). Adenosine kinase deficiency: Three new cases and diagnostic value of hypermethioninemia. Molecular Genetics and Metabolism, 132(1), 38-43.

Boison, D., & Jarvis, M. F. (2020). Adenosine kinase: A key regulator of purinergic physiology. Biochemical Pharmacology, 114321.

Moody, C. L., Funk, A. J., Devine, E., Devore Homan, R. C., Boison, D., McCullumsmith, R. E., & O’Donovan, S. M. (2020). Adenosine kinase expression in the frontal cortex in schizophrenia. Schizophrenia bulletin, 46(3), 690-698.

Gebril, H. M., Rose, R. M., Gesese, R., Emond, M. P., Huo, Y., Aronica, E., & Boison, D. (2020). Adenosine kinase inhibition promotes proliferation of neural stem cells after traumatic brain injury. Brain communications, 2(1), fcaa017.

Sandau, U. S., Yahya, M., Bigej, R., Friedman, J. L., Saleumvong, B., & Boison, D. (2019). Transient use of a systemic adenosine kinase inhibitor attenuates epilepsy development in mice. Epilepsia, 60(4), 615-625.

Cao, W., Yuan, Y., Liu, X., Li, Q., An, X., Huang, Z., ... & Xing, C. (2019). Adenosine kinase inhibition protects against cisplatin-induced nephrotoxicity. American Journal of Physiology-Renal Physiology, 317(1), F107-F115.

Zhang, M., Zeng, X., Yang, Q., Xu, J., Liu, Z., Zhou, Y., ... & Huo, Y. (2018). Ablation of myeloid ADK (adenosine kinase) epigenetically suppresses atherosclerosis in ApoE−/−(apolipoprotein E deficient) mice. Arteriosclerosis, thrombosis, and vascular biology, 38(12), 2780-2792.

Xu, Y., Wang, Y., Yan, S., Yang, Q., Zhou, Y., Zeng, X., ... & Huo, Y. (2017). Regulation of endothelial intracellular adenosine via adenosine kinase epigenetically modulates vascular inflammation. Nature communications, 8(1), 1-16.

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For research use only. Not intended for any clinical use.

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