Rabbit Anti-ADORA1 Recombinant Antibody (
10D12) (V2LY-0725-LY1068)

Name: Rabbit Anti-ADORA1 Recombinant Antibody (10D12)
SKU: V2LY-0725-LY1068
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ADORA1 Recombinant Antibody (10D12) (V2LY-0725-LY1068) in WB

Western Blot
Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate
All lanes: ADORA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 14 kDa
Observed band size: 37 kDa

Rabbit Anti-ADORA1 Recombinant Antibody (10D12) (V2LY-0725-LY1068) in IHC

IHC image of V2LY-0725-LY1068 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

10D12

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human ADORA1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Adenosine A1 Receptor

Entrez Gene ID

134

UniProt ID

P30542

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Biological Process

Activation of MAPKK activity
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
Apoptotic signaling pathway
Cell-cell signaling
Cognition
Detection of temperature stimulus involved in sensory perception of pain
Excitatory postsynaptic potential
Fatty acid homeostasis
G protein-coupled receptor signaling pathway
Inflammatory response
Lipid catabolic process
Negative regulation of acute inflammatory response
Negative regulation of apoptotic process
Negative regulation of cell population proliferation
Negative regulation of circadian sleep/wake cycle, non-REM sleep
Negative regulation of glutamate secretion
Negative regulation of heart contraction
Negative regulation of hormone secretion
Negative regulation of leukocyte migration
Negative regulation of lipid catabolic process
Negative regulation of long-term synaptic depression
Negative regulation of long-term synaptic potentiation
Negative regulation of mucus secretion
Negative regulation of neurotrophin production
Negative regulation of renal sodium excretion
Negative regulation of synaptic transmission, GABAergic
Negative regulation of synaptic transmission, glutamatergic
Negative regulation of systemic arterial blood pressure
Nervous system development
Phagocytosis
Positive regulation of epidermal growth factor-activated receptor activity
Positive regulation of lipid catabolic process
Positive regulation of neuron death
Positive regulation of nucleoside transport
Positive regulation of peptide secretion
Positive regulation of potassium ion transport
Positive regulation of protein dephosphorylation
Positive regulation of systemic arterial blood pressure
Protein targeting to membrane
Regulation of cardiac muscle cell contraction
Regulation of glomerular filtration
Regulation of respiratory gaseous exchange by nervous system process
Regulation of sensory perception of pain
Relaxation of vascular associated smooth muscle
Response to hypoxia
Signal transduction
Temperature homeostasis
Triglyceride homeostasis

Cellular Location

Cell membrane

Topology

Extracellular: 1-10 aa
Helical: 11-33 aa
Cytoplasmic: 34-46 aa
Helical: 47-69 aa
Extracellular: 70-80 aa
Helical: 81-102 aa
Cytoplasmic: 103-123 aa
Helical: 124-146 aa
Extracellular: 147-176 aa
Helical: 177-201 aa
Cytoplasmic: 202-235 aa
Helical: 236-259 aa
Extracellular: 260-267 aa
Helical: 268-292 aa
Cytoplasmic: 293-326 aa

Hohoff, C., Kroll, T., Zhao, B., Kerkenberg, N., Lang, I., Schwarte, K., ... & Deckert, J. (2020). ADORA2A variation and adenosine A 1 receptor availability in the human brain with a focus on anxiety-related brain regions: modulation by ADORA1 variation. Translational psychiatry, 10(1), 1-11.

Wu, X., You, W., Wu, Z., Ye, F., & Chen, S. (2020). Zinc finger protein 91 loss induces cardiac hypertrophy through adenosine A1 receptor down‐regulation under pressure overload status. Journal of Cellular and Molecular Medicine, 24(17), 10189-10201.

Province, H. S., Xiao, C., Mogul, A. S., Sahoo, A., Jacobson, K. A., Piñol, R. A., ... & Reitman, M. L. (2020). Activation of neuronal adenosine A1 receptors causes hypothermia through central and peripheral mechanisms. PloS one, 15(12), e0243986.

Zhang, J., Hou, Y., Du, X. L., Chen, D., Sui, G., Qi, Y., ... & Yang, Y. (2020). ADORA 1-driven brain-sympathetic neuro-adipose connections control body weight and adipose lipid metabolism. Molecular Psychiatry, 1-15.

Choi, S. J., Ryu, E., Lee, S., Huh, S., Shin, Y. S., Kang, B. W., ... & Kang, H. (2019). Adenosine induces EBV lytic reactivation through ADORA1 in EBV-associated gastric carcinoma. International journal of molecular sciences, 20(6), 1286.

Mei, H. F., Poonit, N., Zhang, Y. C., Ye, C. Y., Cai, H. L., Yu, C. Y., ... & Cai, X. H. (2018). Activating adenosine A1 receptor accelerates PC12 cell injury via ADORA1/PKC/KATP pathway after intermittent hypoxia exposure. Molecular and cellular biochemistry, 446(1), 161-170.

Hackett, T. A. (2018). Adenosine A1 receptor mRNA expression by neurons and glia in the auditory forebrain. The Anatomical Record, 301(11), 1882-1905.

Fernández-Dueñas, V., Pérez-Arévalo, A., Altafaj, X., Ferré, S., & Ciruela, F. (2017). Adenosine A1-A2A receptor heteromer as a possible target for early-onset Parkinson's disease. Frontiers in neuroscience, 11, 652.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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