What is the immunogen used to generate this antibody?

This antibody was produced using a recombinant protein corresponding to amino acids 221-326, which maps to an internal C-terminal domain of the human Adenosine A1 Receptor (ADORA1). The recombinant nature of this antibody ensures high specificity and batch consistency.

What sample preparation is recommended for Western blotting of this GPCR?

For transmembrane proteins like ADORA1, we recommend using membrane-enriched protein fractions rather than whole-cell lysates to increase the target protein concentration. It is also important to avoid boiling the samples, as this can cause GPCRs to aggregate; heating at 37-50°C for 10-15 minutes prior to loading is often sufficient.

Mouse Anti-ADORA1 Recombinant Antibody (V2-180006) (CBMAB-A1393-YC)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-180006

Application

WB, IP, IF, ELISA

Immunogen

Raised against amino acids 287-326 mapping at the C-terminus of Adenosine A1-R of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adenosine A1 Receptor

Introduction

ADORA1 is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiolo

Entrez Gene ID

134

UniProt ID

P30542

Alternative Names

Adenosine A1 Receptor; Adenosine A1 Receptor Variant 1; Adenosine A1 Receptor Variant 2; Adenosine Receptor A1; RDC7;

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Biological Process

Activation of MAPKK activity
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
Apoptotic signaling pathway
Cell-cell signaling
Cognition
Detection of temperature stimulus involved in sensory perception of pain
Excitatory postsynaptic potential
Fatty acid homeostasis
G protein-coupled receptor signaling pathway
Inflammatory response
Lipid catabolic process
Negative regulation of acute inflammatory response
Negative regulation of apoptotic process
Negative regulation of cell population proliferation
Negative regulation of circadian sleep/wake cycle, non-REM sleep
Negative regulation of glutamate secretion
Negative regulation of heart contraction
Negative regulation of hormone secretion
Negative regulation of leukocyte migration
Negative regulation of lipid catabolic process
Negative regulation of long-term synaptic depression
Negative regulation of long-term synaptic potentiation
Negative regulation of mucus secretion
Negative regulation of neurotrophin production
Negative regulation of renal sodium excretion
Negative regulation of synaptic transmission, GABAergic
Negative regulation of synaptic transmission, glutamatergic
Negative regulation of systemic arterial blood pressure
Nervous system development
Phagocytosis
Positive regulation of epidermal growth factor-activated receptor activity
Positive regulation of lipid catabolic process
Positive regulation of neuron death
Positive regulation of nucleoside transport
Positive regulation of peptide secretion
Positive regulation of potassium ion transport
Positive regulation of protein dephosphorylation
Positive regulation of systemic arterial blood pressure
Protein targeting to membrane
Regulation of cardiac muscle cell contraction
Regulation of glomerular filtration
Regulation of respiratory gaseous exchange by nervous system process
Regulation of sensory perception of pain
Relaxation of vascular associated smooth muscle
Response to hypoxia
Signal transduction
Temperature homeostasis
Triglyceride homeostasis

Cellular Location

Cell membrane

Topology

Extracellular: 1-10 aa
Helical: 11-33 aa
Cytoplasmic: 34-46 aa
Helical: 47-69 aa
Extracellular: 70-80 aa
Helical: 81-102 aa
Cytoplasmic: 103-123 aa
Helical: 124-146 aa
Extracellular: 147-176 aa
Helical: 177-201 aa
Cytoplasmic: 202-235 aa
Helical: 236-259 aa
Extracellular: 260-267 aa
Helical: 268-292 aa
Cytoplasmic: 293-326 aa

Hohoff, C., Kroll, T., Zhao, B., Kerkenberg, N., Lang, I., Schwarte, K., ... & Deckert, J. (2020). ADORA2A variation and adenosine A 1 receptor availability in the human brain with a focus on anxiety-related brain regions: modulation by ADORA1 variation. Translational psychiatry, 10(1), 1-11.

Wu, X., You, W., Wu, Z., Ye, F., & Chen, S. (2020). Zinc finger protein 91 loss induces cardiac hypertrophy through adenosine A1 receptor down‐regulation under pressure overload status. Journal of Cellular and Molecular Medicine, 24(17), 10189-10201.

Province, H. S., Xiao, C., Mogul, A. S., Sahoo, A., Jacobson, K. A., Piñol, R. A., ... & Reitman, M. L. (2020). Activation of neuronal adenosine A1 receptors causes hypothermia through central and peripheral mechanisms. PloS one, 15(12), e0243986.

Zhang, J., Hou, Y., Du, X. L., Chen, D., Sui, G., Qi, Y., ... & Yang, Y. (2020). ADORA 1-driven brain-sympathetic neuro-adipose connections control body weight and adipose lipid metabolism. Molecular Psychiatry, 1-15.

Choi, S. J., Ryu, E., Lee, S., Huh, S., Shin, Y. S., Kang, B. W., ... & Kang, H. (2019). Adenosine induces EBV lytic reactivation through ADORA1 in EBV-associated gastric carcinoma. International journal of molecular sciences, 20(6), 1286.

Mei, H. F., Poonit, N., Zhang, Y. C., Ye, C. Y., Cai, H. L., Yu, C. Y., ... & Cai, X. H. (2018). Activating adenosine A1 receptor accelerates PC12 cell injury via ADORA1/PKC/KATP pathway after intermittent hypoxia exposure. Molecular and cellular biochemistry, 446(1), 161-170.

Hackett, T. A. (2018). Adenosine A1 receptor mRNA expression by neurons and glia in the auditory forebrain. The Anatomical Record, 301(11), 1882-1905.

Fernández-Dueñas, V., Pérez-Arévalo, A., Altafaj, X., Ferré, S., & Ciruela, F. (2017). Adenosine A1-A2A receptor heteromer as a possible target for early-onset Parkinson's disease. Frontiers in neuroscience, 11, 652.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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