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Rabbit Anti-AEBP2 Recombinant Antibody (V2-180037) (CBMAB-A1443-YC)

Provided herein is a Rabbit monoclonal antibody against Human AE Binding Protein 2. The antibody can be used for immunoassay techniques, such as WB, IP, ChIP.
See all AEBP2 antibodies
Published Data
Rabbit Anti-AEBP2 Recombinant Antibody (D7C6X) (CBMAB-A1443-YC) in WB
Immunoblots for FLAG, SUZ12 (N terminus), EZH2, JARID2, AEBP2, PCL2, EPOP, TRRAP, and β-actin in input (In.) and SUZ12 IPs from WT or Suz12GT/GT ESCs stably expressing FLAG-tagged GFP, SUZ12, SUZ12Δ93, JAZF1-SUZ12, or JAZF1. Data are representative of two independent IPs. ...View More
Tavares, M., Khandelwal, G., Muter, J., Viiri, K., Beltran, M., Brosens, J. J., & Jenner, R. G. (2022). JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation. Cell Reports, 39(9), 110889.
Rabbit Anti-AEBP2 Recombinant Antibody (D7C6X) (CBMAB-A1443-YC) in WB
Western blot showing the indicated protein levels in parental and KO mESCs. Ezh2 KO decreased the total levels of H3K27me3 and increased the H3K27ac. Aebp2 KO didn't exhibit obvious effects on the total levels of tested histone marks. Cell extracts were analyzed via Western blotting using the indicated antibodies, and two biological replicates were performed for each blot. Star indicated a nonspecific band. ...View More
Sun, Z., Tang, Y., Zhang, Y., Fang, Y., Jia, J., Zeng, W., & Fang, D. (2021). Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division. Nature Communications, 12(1), 5941.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-180037
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Leu345 of human AEBP2 protein.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50
CIP1:25

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AE Binding Protein 2
Introduction
DNA-binding transcriptional repressor. May interact with and stimulate the activity of the PRC2 complex, which methylates Lys-9 and Lys-27 residues of histone H3.
Entrez Gene ID
Human158
Mouse11569
Rat315150
Monkey103218722
Hamster100765454
Chicken418184
Cattle537791
UniProt ID
HumanP30566
RatD3ZW08
MouseQ9Z248
HamsterG3HYA2
ChickenA0A1D5PMP0
CattleA4FV57
MonkeyQ4R381
Alternative Names
AE Binding Protein 2; Adipocyte Enhancer-Binding Protein 2; AE-Binding Protein 2;
Function
Acts as an accessory subunit for the core Polycomb repressive complex 2 (PRC2), which mediates histone H3K27 (H3K27me3) trimethylation on chromatin leading to transcriptional repression of the affected target gene. Plays a role in nucleosome localization of the PRC2 complex.
Biological Process
Chromatin organization
Negative regulation of gene expression, epigenetic
Regulation of transcription by RNA polymerase II
Cellular Location
Nucleus. Localizes to chromatin as part of the PRC2 complex.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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