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Rabbit Anti-AGO3 Recombinant Antibody (V2-606901) (CBMAB-R4327-CN)

This product is a rabbit recombinant antibody that recognizes AGO3. The antibody CBNH-002 can be used for immunoassay techniques such as: ELISA.
See all AGO3 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-606901
Antibody Isotype
IgG
Application
WB, IHC

Basic Information

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 721-860 of human Argonaute 3.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
IHC1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 0.05% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target

Full Name
AGO3
Introduction
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
8350
UniProt ID
P68431
Alternative Names
Argonaute 3, RISC Catalytic Component; Argonaute RISC Catalytic Component 3; Eukaryotic Translation Initiation Factor 2C, 3; EIF2C3; HAgo3; Eukaryotic Translation Initiation Factor 2C 3;
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
Biological Process
Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA catabolic process
Negative regulation of gene expression
Positive regulation of gene expression
Positive regulation of NIK/NF-kappaB signaling
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of stem cell proliferation
Regulation of transcription initiation from RNA polymerase II promoter
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway
Cellular Location
P-body
PTM
Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO3 when it is engaged with a TDMD target.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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