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Rabbit Anti-AGO3 Recombinant Antibody (V2-180175) (CBMAB-A1596-YC)

Provided herein is a Rabbit monoclonal antibody against Human Argonaute 3, RISC Catalytic Component. The antibody can be used for immunoassay techniques, such as WB, IP.
See all AGO3 antibodies
Published Data
Rabbit Anti-AGO3 Recombinant Antibody (D15D2) (CBMAB-A1596-YC) in Immunoblot
Following transfection of control siRNA or siDHX9 into KGN cells, AGO3-mediated RISC-associated RNAs were immunoprecipitated using an anti-AGO3 antibody. IgG was used as a control. Co-immunoprecipitated mRNAs were reverse transcribed using a FOXL2-430-R primer binding downstream of the 402C>G site. The cDNA products were used for FOXL2 allele-specific PCR analysis with a FOXL2-279F primer (Appendix Fig S1A), and a representative result obtained by RT-PCR (top) is shown. Quantitative real-time RT-PCR results (middle) are also presented as fold enrichment of FOXL2 mRNAs normalized using the level of GAPDH mRNA. Western blots of immunoprecipitated AGO3 and the inputs are shown in the bottom panel. The data are presented as the mean ± SEM from three independent experiments. Different letters denote statistically significant differences (P < 0.05; Student-Newman-Keuls test). ...View More
Shin, E., Jin, H., Suh, D. S., Luo, Y., Ha, H. J., Kim, T. H., ... & Bae, J. (2020). An alternative miRISC targets a cancer-associated coding sequence mutation in FOXL2. The EMBO journal, 39(24), e104719.
Rabbit Anti-AGO3 Recombinant Antibody (D15D2) (CBMAB-A1596-YC) in WB
Western blot and densitometric analyses showing α1A-Q33-FLAG and α1ACT-Q33-FLAG expression in HEK293 cells treated with four siRNAs or si-C in the presence of miR-3191-5p or NC (n = 6). ...View More
Miyazaki, Y., Du, X., Muramatsu, S. I., & Gomez, C. M. (2016). An miRNA-mediated therapy for SCA6 blocks IRES-driven translation of the CACNA1A second cistron. Science translational medicine, 8(347), 347ra94-347ra94.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Monkey, Rat
Clone
V2-180175
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Val160 of human Argonaute 3 protein.
Host Species
Rabbit
Specificity
Human, Mouse, Monkey, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AGO3
Introduction
AGO3 is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is lo
Entrez Gene ID
Human192669
Mouse214150
Rat313593
Monkey712543
UniProt ID
HumanQ9H9G7
MouseQ8CJF9
RatF1LUS2
MonkeyH2PYM8
Alternative Names
Argonaute 3, RISC Catalytic Component; Argonaute RISC Catalytic Component 3; Eukaryotic Translation Initiation Factor 2C, 3; EIF2C3; HAgo3; Eukaryotic Translation Initiation Factor 2C 3;
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
Biological Process
Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA catabolic process
Negative regulation of gene expression
Positive regulation of gene expression
Positive regulation of NIK/NF-kappaB signaling
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of stem cell proliferation
Regulation of transcription initiation from RNA polymerase II promoter
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway
Cellular Location
P-body
PTM
Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO3 when it is engaged with a TDMD target.
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For research use only. Not intended for any clinical use.

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