Mouse Anti-AGO3 Recombinant Antibody (V2-180177) (CBMAB-A1598-YC)

Name: Mouse Anti-AGO3 Recombinant Antibody (V2-180177)
SKU: CBMAB-A1598-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AGO3 Recombinant Antibody (CBYC-A415) (CBMAB-A1598-YC) in IP

Abundance and length distribution of small RNAs immunoprecipitated with anti-AGO
antibodies. ...View More

Yang, Q., Li, R., Lyu, Q., Hou, L., Liu, Z., Sun, Q., ... & Wu, L. (2019). Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes. Nature communications, 10(1), 3389.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-180177

Application

ICC, IP

Immunogen

Recombinant protein corresponding to amino acids 1-175 of human Ago3.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

70mM tris, pH 8.0, 0.07mM EDTA, 30% glycerol, 31mM glycine

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

AGO3

Introduction

AGO3 is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is lo

Entrez Gene ID

192669

UniProt ID

Q9H9G7

Alternative Names

Argonaute 3, RISC Catalytic Component; Argonaute RISC Catalytic Component 3; Eukaryotic Translation Initiation Factor 2C, 3; EIF2C3; HAgo3; Eukaryotic Translation Initiation Factor 2C 3;

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Biological Process

Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA catabolic process
Negative regulation of gene expression
Positive regulation of gene expression
Positive regulation of NIK/NF-kappaB signaling
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of stem cell proliferation
Regulation of transcription initiation from RNA polymerase II promoter
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway

Cellular Location

P-body

PTM

Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO3 when it is engaged with a TDMD target.

Pan, L., Xu, C., Mei, J., Chen, Y., & Wang, D. (2021). Argonaute 3 (AGO3) promotes malignancy potential of cervical cancer via regulation of Wnt/β-catenin signaling pathway. Reproductive Biology, 21(1), 100479.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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