Sign in or Register   Sign in or Register
  |  

Mouse Anti-AGO3 Recombinant Antibody (V2-180177) (CBMAB-A1598-YC)

Provided herein is a Mouse monoclonal antibody against Human Argonaute 3, RISC Catalytic Component. The antibody can be used for immunoassay techniques, such as ICC, IP.
See all AGO3 antibodies
Published Data
Mouse Anti-AGO3 Recombinant Antibody (CBYC-A415) (CBMAB-A1598-YC) in IP
Abundance and length distribution of small RNAs immunoprecipitated with anti-AGO
antibodies. ...View More
Yang, Q., Li, R., Lyu, Q., Hou, L., Liu, Z., Sun, Q., ... & Wu, L. (2019). Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes. Nature communications, 10(1), 3389.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180177
Antibody Isotype
IgG2a, κ
Application
ICC, IP

Basic Information

Immunogen
Recombinant protein corresponding to amino acids 1-175 of human Ago3.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2
Buffer
70mM tris, pH 8.0, 0.07mM EDTA, 30% glycerol, 31mM glycine
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AGO3
Introduction
AGO3 is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is lo
Entrez Gene ID
192669
UniProt ID
Q9H9G7
Alternative Names
Argonaute 3, RISC Catalytic Component; Argonaute RISC Catalytic Component 3; Eukaryotic Translation Initiation Factor 2C, 3; EIF2C3; HAgo3; Eukaryotic Translation Initiation Factor 2C 3;
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
Biological Process
Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA catabolic process
Negative regulation of gene expression
Positive regulation of gene expression
Positive regulation of NIK/NF-kappaB signaling
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of stem cell proliferation
Regulation of transcription initiation from RNA polymerase II promoter
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway
Cellular Location
P-body
PTM
Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO3 when it is engaged with a TDMD target.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-AGO3 Recombinant Antibody (V2-180177)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare