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Mouse Anti-AGRP Recombinant Antibody (V2-180227) (CBMAB-A1655-YC)

Provided herein is a Mouse monoclonal antibody against Human Agouti Related Neuropeptide. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AGRP antibodies
Published Data
Mouse Anti-AGRP Recombinant Antibody (CBYC-A425) (CBMAB-A1655-YC) in WB
Western blot bands for the AgRP, NPY, POMC, and CART proteins in the different groups. ...View More
Liao, T., Zhang, S. L., Yuan, X., Mo, W. Q., Wei, F., Zhao, S. N., ... & Rong, X. (2020). Liraglutide lowers body weight set point in DIO rats and its relationship with hypothalamic microglia activation. Obesity, 28(1), 122-131.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180227
Antibody Isotype
IgG1
Application
ICC, IHC, IP, WB

Basic Information

Immunogen
Amino acids 64-90 within an internal region of AGRP of mouse origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized from PBS with trehalose
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Agouti Related Neuropeptide
Introduction
AGRP is an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutati
Entrez Gene ID
181
UniProt ID
O00253
Alternative Names
Agouti Related Neuropeptide; AGRT; ART; Agouti Related Protein Homolog (Mouse); Agouti (Mouse) Related Protein; Agouti Related Protein Homolog; Agouti-Related Protein; ASIP2;
Function
Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.
Biological Process
Adult feeding behavior
Circadian rhythm
Eating behavior
Feeding behavior
Hormone-mediated signaling pathway
Long-day photoperiodism
Maternal process involved in female pregnancy
Neuropeptide signaling pathway
Positive regulation of feeding behavior
Regulation of feeding behavior
Response to insulin
Cellular Location
Secreted; Golgi apparatus lumen
Involvement in disease
Obesity (OBESITY): A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Loganathan, N. (2021). Molecular mechanisms involved in the regulation of agouti-related peptide and neuropeptide Y by endocrine disrupting chemical bisphenol A in hypothalamic neurons (Doctoral dissertation, University of Toronto (Canada)).

Qiu, J., Bosch, M. A., Zhang, C., Rønnekleiv, O. K., & Kelly, M. J. (2020). Estradiol protects neuropeptide Y/agouti-related peptide neurons against insulin resistance in females. Neuroendocrinology, 110(1-2), 105-118.

Kalananthan, T., Murashita, K., Rønnestad, I., Ishigaki, M., Takahashi, K., Silva, M. S., ... & Gomes, A. S. (2020). Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon (Salmo salar, L.). Frontiers in physiology, 11, 61.

Jiang, J., Morgan, D. A., Cui, H., & Rahmouni, K. (2020). Activation of hypothalamic AgRP and POMC neurons evokes disparate sympathetic and cardiovascular responses. American Journal of Physiology-Heart and Circulatory Physiology, 319(5), H1069-H1077.

Chen, Y., Essner, R. A., Kosar, S., Miller, O. H., Lin, Y. C., Mesgarzadeh, S., & Knight, Z. A. (2019). Sustained NPY signaling enables AgRP neurons to drive feeding. Elife, 8, e46348.

Wu, J., Zhu, C., Yang, L., Wang, Z., Wang, L., Wang, S., ... & Shu, G. (2017). N-oleoylglycine-induced hyperphagia is associated with the activation of agouti-related protein (AgRP) neuron by cannabinoid receptor type 1 (CB1R). Journal of agricultural and food chemistry, 65(5), 1051-1057.

Chen, S. R., Chen, H., Zhou, J. J., Pradhan, G., Sun, Y., Pan, H. L., & Li, D. P. (2017). Ghrelin receptors mediate ghrelin‐induced excitation of agouti‐related protein/neuropeptide Y but not pro‐opiomelanocortin neurons. Journal of neurochemistry, 142(4), 512-520.

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For research use only. Not intended for any clinical use.

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