Mouse Recombinant AGRP protein, hFc Tag-1 (V2LY-0526-LY7784)

Name: Mouse Recombinant AGRP protein, hFc Tag-1
SKU: V2LY-0526-LY7784
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

hFc Tag

Protein Construction

This product is Mouse Recombinant AGRP protein, hFc Tag consist of Amino Acid: 1-131 and predicts a molecular mass of 39.4 kDa.

Molecule Mass

39.4 kDa

Sequence

Amino Acid: 1-131

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MES, NaCl, Glycerol, Tween20, Trehalose

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Agouti Related Neuropeptide

Biological Process

Adult feeding behavior
Circadian rhythm
Eating behavior
Feeding behavior
Hormone-mediated signaling pathway
Long-day photoperiodism
Maternal process involved in female pregnancy
Neuropeptide signaling pathway
Positive regulation of feeding behavior
Regulation of feeding behavior
Response to insulin

Cellular Location

Secreted; Golgi apparatus lumen

Involvement in disease

Obesity (OBESITY): A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Loganathan, N. (2021). Molecular mechanisms involved in the regulation of agouti-related peptide and neuropeptide Y by endocrine disrupting chemical bisphenol A in hypothalamic neurons (Doctoral dissertation, University of Toronto (Canada)).

Qiu, J., Bosch, M. A., Zhang, C., Rønnekleiv, O. K., & Kelly, M. J. (2020). Estradiol protects neuropeptide Y/agouti-related peptide neurons against insulin resistance in females. Neuroendocrinology, 110(1-2), 105-118.

Kalananthan, T., Murashita, K., Rønnestad, I., Ishigaki, M., Takahashi, K., Silva, M. S., ... & Gomes, A. S. (2020). Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon (Salmo salar, L.). Frontiers in physiology, 11, 61.

Jiang, J., Morgan, D. A., Cui, H., & Rahmouni, K. (2020). Activation of hypothalamic AgRP and POMC neurons evokes disparate sympathetic and cardiovascular responses. American Journal of Physiology-Heart and Circulatory Physiology, 319(5), H1069-H1077.

Chen, Y., Essner, R. A., Kosar, S., Miller, O. H., Lin, Y. C., Mesgarzadeh, S., & Knight, Z. A. (2019). Sustained NPY signaling enables AgRP neurons to drive feeding. Elife, 8, e46348.

Wu, J., Zhu, C., Yang, L., Wang, Z., Wang, L., Wang, S., ... & Shu, G. (2017). N-oleoylglycine-induced hyperphagia is associated with the activation of agouti-related protein (AgRP) neuron by cannabinoid receptor type 1 (CB1R). Journal of agricultural and food chemistry, 65(5), 1051-1057.

Chen, S. R., Chen, H., Zhou, J. J., Pradhan, G., Sun, Y., Pan, H. L., & Li, D. P. (2017). Ghrelin receptors mediate ghrelin‐induced excitation of agouti‐related protein/neuropeptide Y but not pro‐opiomelanocortin neurons. Journal of neurochemistry, 142(4), 512-520.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AGRP protein, hFc Tag (CAT#: V2LY-0526-LY1960)

Human Recombinant AGRP protein, hFc Tag-1 (CAT#: V2LY-0526-LY1961)

Human Recombinant AGRP protein, His Tag (CAT#: V2LY-0526-LY1962)

Mouse Recombinant AGRP protein, hFc Tag (CAT#: V2LY-0526-LY7783)

Mouse Recombinant AGRP protein, His Tag (CAT#: V2LY-0526-LY7785)

Rhesus Recombinant AGRP protein, Fc Tag (CAT#: V2LY-0526-LY9643)

Mouse Agrp ELISA Kit (CAT#: V2LY-0626-LY1273)

Human AGRP ELISA Kit (CAT#: V2LY-0626-LY1689)

Mouse Agrp ELISA Kit (2) (CAT#: V2LY-0626-LY2003)

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Mouse Recombinant AGRP protein, His Tag (CAT#: V2LY-0526-LY7785)

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Human Recombinant AGRP protein, hFc Tag (CAT#: V2LY-0526-LY1960)

Rhesus Recombinant AGRP protein, Fc Tag (CAT#: V2LY-0526-LY9643)

Mouse Anti-AGRP Recombinant Antibody (V2-180226) (CAT#: CBMAB-A1654-YC)

Mouse Agrp ELISA Kit (CAT#: V2LY-0626-LY1273)

Mouse Recombinant AGRP protein, hFc Tag (CAT#: V2LY-0526-LY7783)

Mouse Anti-AGRP Recombinant Antibody (V2-180227) (CAT#: CBMAB-A1655-YC)

Rat Anti-AGRP Recombinant Antibody (V2-180223) (CAT#: CBMAB-A1649-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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